Protein adsorption to oligo(ethylene glycol) self-assembled monolayers: Experiments with fibrinogen, heparinized plasma, and serum
Protein adsorption to oligo(ethylene glycol) self-assembled monolayers: Experiments with fibrinogen, heparinized plasma, and serum
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DOI：
10.1163/156856201316883421
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发表时间：
2001-01-01
影响因子：
3.6
通讯作者：
Tengvall, P
中科院分区：
文献类型：
--
作者：
Benesch, J;Svedhem, S;Tengvall, P
低蛋白质吸附被认为对血液接触材料有利，为此目的，通常将基于乙二醇（EG）的聚合物附着在表面上。在本研究中，纤维蛋白原，血清和血浆的吸附进行了研究，椭圆偏振法上一系列定义明确的寡（EG）终止的烷硫醇自组装金。用通式结构HS-（CH 2）（15）-CONH-EG（n）的化合物制备层，其中n = 2、4和6。以甲氧基封端的三（EG）十一烷醇和羟基封端的十六烷醇自组装膜（SAMs）为参照，结果表明，纤维蛋白原的吸附量与实验条件有关，单一蛋白质溶液中纤维蛋白原的吸附量较小，而血清和血浆中的蛋白质吸附量较大。纤维蛋白原和血浆的吸附随着EG重复数的增加而降低，并且具有温度依赖性。显着减少血清吸附到甲氧基三（EG）比六（EG）和更多的蛋白质仍然在后者的表面上孵育后，在十二烷基硫酸钠（SDS）溶液中，表明松散的蛋白质结合到甲氧基封端的表面。所有表面吸附补体因子3（C3）从血清和血浆，虽然没有观察到表面介导的补体激活。本研究指出了一个仔细选择的蛋白质模型系统的重要性，然后才可以作出关于蛋白质排斥性能的潜在表面的一般性陈述。
Low protein adsorption is believed advantageous for blood-contacting materials and ethylene glycols (EG)-based polymeric compounds are often attached to surfaces for this purpose. In the present study, the adsorption of fibrinogen, serum, and plasma were studied by ellipsometry on a series of well-defined oligo(EG) terminated alkane-thiols self-assembled on gold. The layers were prepared with compounds of the general structure HS-(CH2)(15)-CONH-EG(n), where n = 2, 4, and 6. Methoxy-terminated tri(EG) undecanethiol and hydroxyl-terminated hexadecanethiol self-assembled monolayers (SAMs) were used as references.The results clearly demonstrate that the adsorption depends on the experimental conditions with small amounts of fibrinogen adsorbing from a single protein solution, but larger amounts of proteins from serum and plasma. The adsorption of fibrinogen and blood plasma decreased with an increasing number of EG repeats and was temperature-dependent. Significantly less serum adsorbed to methoxy tri(EG) than to hexa(EG) and more proteins remained on the latter surface after incubation in a sodium dodecyl sulfate (SDS) solution, indicating a looser protein binding to the methoxy-terminated surface. All surfaces adsorbed complement factor 3 (C3) from serum and plasma, although no surface-mediated complement activation was observed. The present study points to the importance of a careful choice of the protein model system before general statements regarding the protein repellant properties of potential surfaces can be made.