Brittle Culm 12, a dual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice.
Brittle Culm 12, a dual-targeting kinesin-4 protein, controls cell-cycle progression and wall properties in rice.
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DOI：
10.1111/j.1365-313x.2010.04238.x
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发表时间：
2010-07
期刊：
影响因子：
--
通讯作者：
Zhou Y
中科院分区：
文献类型：
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作者：
Zhang M;Zhang B;Qian Q;Yu Y;Li R;Zhang J;Liu X;Zeng D;Li J;Zhou Y
驱动蛋白是由一个大的基因家族编码，参与植物发育的许多基本过程。然而，在水稻中功能鉴定的驱动蛋白的数量非常有限。在这里，我们报告了编码驱动蛋白-4蛋白的基因Brittle Culm 12（BC 12）的功能特征。BC 12突变体显示出由于纤维素微纤丝取向和壁组成的改变而导致的细胞数量显著减少和脆性的侏儒症。BC 12主要在经历细胞分裂和次生壁增厚的组织中表达。体外生物化学分析证实BC 12是一种真正的马达蛋白。这种蛋白质存在于细胞核和细胞质中，并在细胞分裂过程中与微管阵列相关。有丝分裂微管阵列比较，流式细胞仪分析和表达测定根尖细胞中的细胞周期蛋白依赖性激酶（CDK）复合物表明，细胞周期进程的影响，在bc 12突变体。基于酵母双杂交和微阵列数据，BC 12很可能受CDKA;3调控。因此，BC 12作为双靶向驱动蛋白发挥功能，并与单子叶植物水稻的细胞周期进程、纤维素微纤丝沉积和细胞壁组成有关。
Kinesins are encoded by a large gene family involved in many basic processes of plant development. However, the number of functionally identified kinesins in rice is very limited. Here, we report the functional characterization of Brittle Culm12 (BC12), a gene encoding a kinesin-4 protein. bc12 mutants display dwarfism resulting from a significant reduction in cell number and brittleness due to an alteration in cellulose microfibril orientation and wall composition. BC12 is expressed mainly in tissues undergoing cell division and secondary wall thickening. In vitro biochemical analyses verified BC12 as an authentic motor protein. This protein was present in both the nucleus and cytoplasm and associated with microtubule arrays during cell division. Mitotic microtubule array comparison, flow cytometric analysis and expression assays of cyclin-dependent kinase (CDK) complexes in root-tip cells showed that cell-cycle progression is affected in bc12 mutants. BC12 is very probably regulated by CDKA;3 based on yeast two-hybrid and microarray data. Therefore, BC12 functions as a dual-targeting kinesin protein and is implicated in cell-cycle progression, cellulose microfibril deposition and wall composition in the monocot plant rice.