Using Drosophila larvae to study epidermal wound closure and inflammation.
Using Drosophila larvae to study epidermal wound closure and inflammation.
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DOI:
10.1007/978-1-62703-505-7_26
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发表时间:
2013
期刊:
影响因子:
--
通讯作者:
Galko, Michael J
中科院分区:
文献类型:
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作者:
Burra, Sirisha;Wang, Yan;Brock, Amanda R;Galko, Michael J
This methods chapter describes two methods for creating epithelial wounds in Drosophila larvae: pinch and puncture wounding. It also covers protocols for visualizing epithelial wounds, either in a dissected whole mount preparation or, using transgenic reporter larvae, in a live whole mount preparation. Finally, useful transgenic lines for live genetic screening of genes required for wound closure or inflammation are described.