Arabidopsis ENHANCER OF SHOOT REGENERATION (ESR)1 and ESR2 regulate in vitro shoot regeneration and their expressions are differentially regulated
Arabidopsis ENHANCER OF SHOOT REGENERATION (ESR)1 and ESR2 regulate in vitro shoot regeneration and their expressions are differentially regulated
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DOI：
10.1016/j.plantsci.2011.03.007
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发表时间：
2011-07-01
期刊：
影响因子：
5.2
通讯作者：
Banno, Hiroharu
中科院分区：
文献类型：
--
作者：
Matsuo, Naoki;Makino, Miho;Banno, Hiroharu
拟南芥中的ESR 1和ESR 2基因在离体芽再生中起着重要的作用。本研究利用ESR 1和ESR 2的突变体和启动子-报告基因系统，研究了ESR 1和ESR 2在再生过程中的功能和表达模式。esr 1和esr 2双突变体的下胚轴不定芽再生效率显著下降，esr 1单突变体对不定芽再生的影响虽然远小于esr 2单突变体，尤其是对根的不定芽再生，但二者的影响是相加的。我们发现，ESR 1最初表达后1天转移到芽诱导培养基（SIM），与4天ESR 2的表达。这些结果表明，ESR 1和ESR 2在芽再生中的功能并不是多余的，即使它们编码相似的转录因子，并且ESR 2基因被ESR 1编码区取代补充了ESR 2突变。我们还发现，ESR 1的表达被定位到少数细胞在侧根分生组织（LRM）样结构，和ESR 1表达细胞出现增殖，形成芽顶端分生组织（SAM）样结构。因此，ESR 1可能参与了组织培养中LRM向SAM的转化。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
The Arabidopsis ENHANCER OF SHOOT REGENERATION (ESR)1 and ESR2 genes are thought to play critical roles in in vitro shoot regeneration. In this study, we investigated the functions and expression patterns of ESR1 and ESR2 during shoot regeneration by using their mutants and promoter-reporter systems. Shoot regeneration efficiencies of esr1 esr2 double mutants from hypocotyl explants decreased drastically; although the effects on shoot regeneration of the esr1 single mutation were much less marked than those of the esr2 single mutation, especially from root explants, their effects were additive. We found that ESR1 was initially expressed 1 day after transfer onto shoot-inducing medium (SIM), compared with 4 days for ESR2 expression. These results suggest that the functions of ESR1 and ESR2 in shoot regeneration are not redundant, even though they encode similar transcription factors and the ESR2 gene substituted with an ESR1 coding region complements the esr2 mutation. We also found that ESR1 expression was localized to a small number of cells in the lateral root meristem (LRM)-like structures, and the ESR1-expressing cells appeared to proliferate to form shoot apical meristem (SAM)-like structures. Thus, ESR1 may be involved in the change of LRM to SAM in tissue culture. (C) 2011 Elsevier Ireland Ltd. All rights reserved.