Silica spheres coated with C18-modified gold nanoparticles for capillary LC and pressurized CEC separations
Silica spheres coated with C18-modified gold nanoparticles for capillary LC and pressurized CEC separations
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涂有 C18 修饰金纳米颗粒的二氧化硅球，用于毛细管 LC 和加压 CEC 分离
DOI：
10.1002/elps.200900375
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发表时间：
2010-02-01
期刊：
影响因子：
2.9
通讯作者：
Yan, Chao
中科院分区：
文献类型：
--
作者：
Qu, Qishu;Peng, Shengwei;Yan, Chao
1.3 μ m的无孔单分散二氧化硅球。用金纳米颗粒（AuNPs）涂覆，随后用正十八烷基乙醇涂覆。通过透射电子显微镜分析，二氧化硅球上的AuNP的平均直径被确定为12 nm。以一组非极性多环芳烃为目标物，研究了自组装正十八烷基乙基金纳米粒子（C18-AuNPs-SiO（2））的色谱和电色谱性能。固定相似乎显示出特征性的反相行为。与毛细管液相色谱相比，加压CEC（pCEC）具有更高的分离效率和更短的分离时间。在pCEC模式下，苯的最大柱效约为2.5 × 10（5）板/m，分离时间小于18 min，而在CLC模式下，苯的最大柱效约为66507板/m，分析时间约为100 min。C18-AuNPs-SiO（2）固定相在极高和极低pH条件下的化学稳定性试验表明，该固定相在pH 12和1下至少稳定60 h。结果表明，C18-AuNPs-SiO（2）具有较高的刚性，能承受较高的填充压力，可作为CLC和pCEC的良好固定相。
Nonporous monodispersed silica spheres of 1.3 mu m. were coated with gold nanoparticles (AuNPs) and subsequently coated with n-octadecanethiol. By transmission electron microscopy analysis, the average diameter of the AuNPs on the silica spheres was determined to be 12 nm. The chromatographic and electrochromatographic properties of self-assembled n-octadecanethiol AuNP-coated silica microspheres (C18-AuNPs-SiO(2)) were investigated using a group of nonpolar PAHs. The stationary phase appears to display a characteristic reversed-phase behavior. Higher separation efficiency and shorter separation times were obtained using pressurized CEC (pCEC) compared with capillary LC (CLC). A maximum column efficiency of about 2.5 x 10(5) plates per meter and less than 18 min separation time for benzene were obtained in pCEC while only 66 507 plates per meter and an analysis time of nearly 100 min were observed in CLC mode. A chemical stability test of the C18-AuNPs-SiO(2) stationary phase under extremely high and low pH conditions demonstrated that it is stable at pH 12 and 1 for at least 60 h. The results confirm that C18-AuNPs-SiO(2) possesses a high rigidity to withstand high packing pressures and can be used as a good stationary phase for CLC and pCEC.