Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold.
Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold.
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新型聚（乳酸）/纳米β-磷酸三钙支架的降解和成骨潜力
DOI：
10.2147/ijn.s38127
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发表时间：
2012
影响因子：
8
通讯作者：
Dong J
中科院分区：
文献类型：
--
作者：
Cao L;Duan PG;Wang HR;Li XL;Yuan FL;Fan ZY;Li SM;Dong J
本研究旨在通过体外降解和体内异位骨形成模型研究纳米级β-磷酸三钙（β-TCP）对聚乳酸（PLA）复合支架生物性能的影响。以微粒径β-TCP （m - β-TCP）为原料，采用湿法研磨法制备纳米级β-TCP (n - β-TCP)，并采用冷冻干燥法制备含有0、10、30、50 wt% n - β-TCP或30 wt% m - β-TCP的复合支架。在26周的水解期间，通过监测微观结构、pH值、重量和抗压强度的变化来评估降解情况。将复合支架加工成块状，加载重组人骨形态发生蛋白-2 （rhBMP-2）后植入家兔肌袋。植入后2周、4周和8周，根据组织学和免疫组织化学分析评估新骨形成。体外实验结果表明，nβ-TCP的缓冲作用强于mβ-TCP，其缓冲作用与nβ-TCP含量呈正相关。体内实验结果表明，nβ-TCP增强了支架的骨导电性。虽然含有30% nβ-TCP的复合支架的骨导电性与50% nβ-TCP相似，但其力学性能优于含有50% nβ-TCP的复合支架。本研究支持含有30% nβ-TCP的复合支架作为一种有前景的骨再生支架的潜在应用。
The purpose of this study was to investigate the influence of nano-sized β-tricalcium phosphate (β-TCP) on the biological performance of poly (lactic acid) (PLA) composite scaffolds by using in vitro degradation and an in vivo model of heterotopic bone formation. Nano-sized β-TCP (nβ-TCP) was prepared with a wet grinding method from micro-sized β-TCP (mβ-TCP), and composite scaffolds containing 0, 10, 30, or 50 wt% nβ-TCP or 30 wt% mβ-TCP were generated using a freeze-drying method. Degradation was assessed by monitoring changes in microstructure, pH, weight, and compressive strength over a 26-week period of hydrolysis. Composite scaffolds were processed into blocks, and implanted into muscular pockets of rabbits after loading with recombinant human bone morphogenetic protein-2 (rhBMP-2). New bone formation was evaluated based on histological and immunohistochemical analysis 2, 4, and 8 weeks after implantation. The in vitro results indicated that the buffering effect of nβ-TCP was stronger than mβ-TCP, which was positively correlated with the content of nβ-TCP. The in vivo findings demonstrated that nβ-TCP enhanced the osteoconductivity of the scaffolds. Although composite scaffolds containing 30% nβ-TCP exhibited similar osteoconductivity to 50% nβ-TCP, they had better mechanical properties than the 50% nβ-TCP scaffolds. This study supports the potential application of a composite scaffold containing 30% nβ-TCP as a promising scaffold for bone regeneration.