HUMAN AND BOVINE SEROTYPE G8 ROTAVIRUSES MAY BE DERIVED BY REASSORTMENT
HUMAN AND BOVINE SEROTYPE G8 ROTAVIRUSES MAY BE DERIVED BY REASSORTMENT
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DOI：
10.1007/bf01309632
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发表时间：
1992-01-01
影响因子：
2.7
通讯作者：
GERNA, G
中科院分区：
文献类型：
--
作者：
BROWNING, GF;SNODGRASS, DR;GERNA, G
应用基因组杂交技术研究了人和牛血清型G8轮状病毒的起源和亲缘关系。使用人G8轮状病毒69 M（在印度尼西亚分离）和HAL 1271（在芬兰分离）以及牛轮状病毒KK 3（G 10）和NCDV（G 6）的放射性标记mRNA作为探针。用聚丙烯酰胺凝胶分离法检测了探针与人和牛轮状病毒基因组RNA的液体杂交产物，包括牛G8轮状病毒678（苏格兰分离）和另外两种芬兰人G8轮状病毒HAL 1166和HAL 8590。芬兰人G8轮状病毒的基因组与牛G6和G10轮状病毒的基因组相似。印度尼西亚人G8和牛G8病毒彼此之间或与其他牛轮状病毒和人轮状病毒之间都没有高水平的相似性。因此，这三种流行病学上不同的G8轮状病毒具有不同的起源，并且可能通过与第三种（迄今未知）宿主种属的轮状病毒重配而衍生。芬兰分离株和牛分离株NCDV之间的相似性表明，它们最近发生了分化，这些人G8轮状病毒可能来源于人畜共患感染，或者来源于已用于接种芬兰儿童的牛源性五种轮状病毒疫苗。
The origin of, and relationship between human and bovine serotype G 8 rotaviruses were investigated by genomic hybridisation. Radiolabelled mRNAs of human G 8 rotaviruses 69 M (isolated in Indonesia) and HAL 1271 (isolated in Finland), and bovine rotaviruses KK 3 (G 10) and NCDV (G 6), were used as probes. The products of liquid hybridisation between the probes and the genomic RNA of human and bovine rotaviruses, including bovine G 8 rotavirus 678 (isolated in Scotland) and two other Finnish human G 8 rotaviruses HAL 1166 and HAL 8590, were examined by separation in polyacrylamide gels. The genomes of Finnish human G 8 rotaviruses were similar to those of bovine G 6 and G 10 rotaviruses. Neither Indonesian human G 8 nor bovine G 8 viruses had high levels of similarity to each other or to other bovine and human rotaviruses. Thus these three epidemiologically distinct G 8 rotaviruses have different origins and may be derived by reassortment with rotaviruses of a third, as yet unknown, host species. The similarity between the Finnish isolates and the bovine isolate NCDV suggests that they have diverged recently and that these human G 8 rotaviruses may be derived from a zoonotic infection, or alteratively, from the five rotavirus vaccine of bovine origin which has been used to vaccinate Finnish children.