PCR detection of hemolysin (vhh) gene in Vibrio harveyi
PCR detection of hemolysin (vhh) gene in Vibrio harveyi
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DOI：
10.2323/jgam.50.137
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发表时间：
2004-06-01
影响因子：
1.2
通讯作者：
Hedreyda, CT
中科院分区：
文献类型：
--
作者：
Conejero, MJU;Hedreyda, CT
哈维弧菌溶血素基因（vhh）编码一种与鱼类和贝类致病性有关的毒力因子，可能成为物种检测或菌株分化的目标。为该基因设计的引物用于检测不同寄主的哈维氏病毒株。在所测试的4个引物中，有1个引物可以用所研究的11株哈维氏病毒的模板扩增出预期的基因片段。因此，检测溶血素基因的存在可作为对鱼和虾具有潜在致病性的哈氏弧菌分离株的合适检测标记。
The Vibrio harveyi hemolysin gene (vhh), which encodes for a virulence factor involved in pathogenicity to fish and shellfish species, may be targeted for species detection or strain differentiation. Primers designed for this gene were used in detection studies of V harveyi strains from various hosts. One primer set among four tested, could amplify the expected gene fragment in PCR using templates from all 11 V harveyi strains studied. Detection of the presence of the hemolysin gene could therefore serve as a suitable detection marker of Vibrio harveyi isolates potentially pathogenic to fish and shrimps.