Neuropeptide substance P stimulates the formation of osteoclasts via synovial fibroblastic cells.
Neuropeptide substance P stimulates the formation of osteoclasts via synovial fibroblastic cells.
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DOI：
10.2504/kds.59.119_1
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发表时间：
2005-02
影响因子：
3.1
通讯作者：
T. Matayoshi;T. Goto;E. Fukuhara;H. Takano;Shigeru Kobayashi;Tetsu Takahashi
中科院分区：
文献类型：
--
作者：
T. Matayoshi;T. Goto;E. Fukuhara;H. Takano;Shigeru Kobayashi;Tetsu Takahashi
本研究旨在探讨神经肽P物质(SP)对滑膜成纤维细胞形成破骨细胞的影响。大鼠膝关节滑膜成纤维细胞表达SP受体、神经激肽-1受体(NK(1)-R)。加入SP可促进滑膜成纤维细胞的增殖，此作用可被SP或NK(1)-R拮抗剂抑制。逆转录聚合酶链式反应和免疫荧光染色显示，加入SP后，滑膜成纤维细胞核因子kappaB受体激活剂配体(RANKL)的表达增加。滑膜成纤维细胞经SP孵育后，骨保护素表达降低。在滑膜成纤维细胞与大鼠外周血单核细胞共培养中，SP可促进破骨细胞的生成。这些结果提示，关节腔内的SP可能通过增加滑膜RANKL的表达而引起滑膜肥大和诱导破骨细胞形成增多。
The present study was designed to evaluate the effects of neuropeptide substance P (SP) on the formation of osteoclasts via synovial fibroblastic cells. Synovial fibroblastic cells derived from rat knee joint expressed the SP receptor, neurokinin-1 receptor (NK(1)-R). The addition of SP stimulated the proliferation of synovial fibroblastic cells and this effect was inhibited by SP or NK(1)-R antagonists. Increased expression of the receptor activator of nuclear factor kappaB ligand (RANKL) in synovial fibroblastic cells after the addition of SP was demonstrated by reverse transcriptase-polymerase chain reaction and immunofluorescence staining. Osteoprotegerin expression in synovial fibroblastic cells was decreased after incubation with SP. In co-cultures of synovial fibroblastic cells and rat peripheral blood monocytes, SP stimulated osteoclastogenesis. These results suggest that SP in the joint cavity may cause both hypertrophy of the synovium and induction of increased osteoclast formation through the increased expression of RANKL in the synovium.