Stimulatory Effect of Brazilian Propolis on Hair Growth through Proliferation of Keratinocytes in Mice
Stimulatory Effect of Brazilian Propolis on Hair Growth through Proliferation of Keratinocytes in Mice
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DOI：
10.1021/jf503184s
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发表时间：
2014-12-10
影响因子：
6.1
通讯作者：
Kobayashi, Ken
中科院分区：
文献类型：
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作者：
Miyata, Shota;Oda, Yozo;Kobayashi, Ken
蜂胶是一种天然蜂巢产品，可用于治疗皮肤病，如皮肤擦伤、烧伤和痤疮。在这项研究中，我们研究了蜂胶是否会刺激小鼠的毛发生长。乙醇提取的蜂胶含有多种生理活性物质，如咖啡酸和山奈酚，可刺激剃光后皮肤的毛发生长初期诱导。发生毛发生长初期诱导时，毛囊 (HF) 形状、凸起中的毛发干细胞、毛球中增殖的毛发基质角质形成细胞或毛乳头中多功能蛋白聚糖的定位没有任何可检测到的异常。蜂胶治疗还刺激了 HF 中脱毛后皮肤生长期晚期的毛基质角质形成细胞迁移到毛干中。含有生长期 HF 的皮肤器官型培养显示蜂胶可显着刺激毛发基质角质形成细胞增殖。此外，蜂胶促进表皮角质形成细胞的增殖。这些结果表明蜂胶通过诱导毛发角质形成细胞增殖来刺激毛发生长。
Propolis is a natural honeybee hive product with the potential for use in the treatment of dermatological conditions, such as cutaneous abrasions, burns, and acne. In this study, we investigated whether propolis stimulates hair growth in mice. Ethanol-extracted propolis, which contains various physiologically active substances such as caffeic acid and kaempferol, stimulated anagen induction in shaved back skin. Anagen induction occurred without any detectable abnormalities in the shape of the hair follicles (HFs), hair stem cells in the bulge, proliferating hair matrix keratinocytes in the hair bulb, or localization of versican in the dermal papilla. Propolis treatment also stimulated migration of hair matrix keratinocytes into the hair shaft in HFs during late anagen in the depilated back skin. Organotypic culture of skin containing anagen stage HFs revealed significant stimulation of hair matrix keratinocyte proliferation by propolis. Furthermore, propolis facilitated the proliferation of epidermal keratinocytes. These results indicate that propolis stimulates hair growth by inducing hair keratinocyte proliferation.