Genetic diversity and tissue compartmentalization of the hepatitis C virus genome in blood mononuclear cells, liver, and serum from chronic hepatitis C patients
Genetic diversity and tissue compartmentalization of the hepatitis C virus genome in blood mononuclear cells, liver, and serum from chronic hepatitis C patients
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DOI：
10.1128/jvi.72.2.1640-1646.1998
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发表时间：
1998-02-01
影响因子：
5.4
通讯作者：
Carreno, V
中科院分区：
文献类型：
--
作者：
Navas, S;Martín, J;Carreno, V
通过对4例慢性丙型肝炎患者的血清、肝组织和外周血单核细胞（PBMC）配对样本中获得的HCV基因组进行克隆和测序，分析了丙型肝炎病毒（HCV）高变区1的遗传变异程度，在复杂性水平上，血清中的遗传变异高于肝组织或PBMC中的遗传变异（从每种类型的组织获得的不同序列的数量）以及在每种组织内的所有序列对之间的遗传距离水平（通过Student t检验比较;两名患者P < 0.001，另一名患者P < 0.01），三种组织中的病毒基因组谱不同，如系统发育分析中根据其起源组织对序列进行分离以及对从不同组织获得的序列之间观察到的平均遗传距离进行统计分析所示（P < 0.001），但肝组织和外周血单个核细胞序列之间的相关性比血清序列之间的相关性更密切。
The degree of genetic variability in the hypervariable region 1 of hepatitis C virus (HCV) was analyzed by cloning and sequencing HCV genomes obtained in paired samples of serum, liver tissue, and peripheral blood mononuclear cells (PBMC) from four chronic hepatitis C patients, Genetic variability in serum was higher than in liver tissue or PBMC at the level of complexity (the number of different sequences obtained from each type of tissue) as well as at the level of genetic distance between all pairs of sequences within each tissue (compared by the Student t test; P < 0.001 for two patients and P < 0.01 for another), The spectrum of viral genomes differed among the three types of tissue, as shown by segregation of sequences according to their tissue of origin in phylogenetic analysis and by statistical analysis of mean genetic distances observed between sequences obtained from different tissues (P < 0.001), but sequences from liver tissue and PBMC were more closely related to each other than to those from serum.