Membrane shape modulates transmembrane protein distribution.
Membrane shape modulates transmembrane protein distribution.
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DOI：
10.1016/j.devcel.2013.12.012
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发表时间：
2014-01-27
影响因子：
11.8
通讯作者：
Bassereau, Patricia
中科院分区：
文献类型：
--
作者：
Aimon, Sophie;Callan-Jones, Andrew;Berthaud, Alice;Pinot, Mathieu;Toombes, Gilman E. S.;Bassereau, Patricia
尽管膜形状在整个细胞中变化很大，但由于细胞中同时发生许多分选机制，膜曲率对跨膜蛋白靶向的贡献尚不清楚。为了分离膜形状的影响，使用含有钾通道KvAP或水通道AQP0的细胞大小的巨型单层囊泡（GUVs）形成半径可控的膜纳米管。AQP0在扁平膜和弯曲膜中的浓度差异不大，而KvAP在管状膜中富集，且在弯曲程度越高的膜中富集程度越高。FRAP测量结果表明，这两种蛋白都可以自由扩散通过管和GUV之间的颈部，并利用热力学分选模型表征了每种蛋白对膜形状和刚度的影响。本研究确立了膜形状对靶向跨膜蛋白的重要性，并为确定膜蛋白的有效形状和柔韧性提供了一种方法。
Although membrane shape varies greatly throughout the cell, the contribution of membrane curvature to transmembrane protein targeting is unknown due to the numerous sorting mechanisms taking place concurrently in cells. To isolate the effect of membrane shape, cellsized Giant Unilamellar Vesicles (GUVs) containing either the potassium channel, KvAP, or water channel, AQP0, were used to form membrane nanotubes with controlled radii. While the AQP0 concentrations in flat and curved membranes were indistinguishable, KvAP was enriched in the tubes, with greater enrichment in more highly curved membranes. FRAP measurements showed that both proteins could freely diffuse through the neck between the tube and GUV, and the effect of each protein on membrane shape and stiffness was characterized using a thermodynamic sorting model. This study establishes the importance of membrane shape for targeting transmembrane proteins, and provides a method for determining the effective shape and flexibility of membrane proteins.
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影响因子：
2.4
作者：
Bozic, B;Kralj-Iglic, V;Svetina, S
通讯作者：
Svetina, S
DOI：
10.1103/physreve.78.041901
发表时间：
2008-10
期刊：
Physical review. E, Statistical, nonlinear, and soft matter physics
影响因子：
--
作者：
Reeves D;Ursell T;Sens P;Kondev J;Phillips R
通讯作者：
Phillips R
DOI：
10.1051/jphys:01986004703050700
发表时间：
1986-03-01
期刊：
JOURNAL DE PHYSIQUE
影响因子：
--
作者：
LEIBLER, S
通讯作者：
LEIBLER, S
影响因子：
15
作者：
Berthaud, Alice;Manzi, John;Mangenot, Stephanie
通讯作者：
Mangenot, Stephanie
影响因子：
15
作者：
Ramadurai, Sivaramakrishnan;Holt, Andrea;Poolman, Bert
通讯作者：
Poolman, Bert