Production and identification of inthomycin B produced by a deep-sea sediment-derived Streptomyces sp YB104 based on cultivation-dependent approach
Production and identification of inthomycin B produced by a deep-sea sediment-derived Streptomyces sp YB104 based on cultivation-dependent approach
复制标题
基于培养依赖性方法，深海沉积物来源的链霉菌 YB104 产生的茵霉素 B 的生产和鉴定
DOI：
10.1007/s00284-018-1469-1
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发表时间：
2018
影响因子：
2.6
通讯作者：
Wang Hong
中科院分区：
文献类型：
--
作者：
Wu Qihao;Zhang Gaiyun;Wang Bixia;Li Xin;Yue Shaopeng;Chen Jianwei;Zhang Huawei;Wang Hong
我们小组的天然产物发现计划利用深海沉积物来源的微生物，并采用生物活性指导分离程序和一种菌株多种化合物（OSMAC）方法来筛选生物活性天然产物，用于医药和农业工业的实际应用。采用OSMAC策略通过改变培养条件来刺激次级代谢产物的产生。本论文通过改变培养基类型，采用培养依赖性方法，从海洋来源的链霉菌中发现了一种生物活性化合物，命名为inthomycin B（1）。YB104。该化合物的特点是基于广泛的光谱分析和比较，在报道的文献。对丝裂霉素B的定量分析表明，丝裂霉素B对链霉菌P. YB 104产生中等产量的茵丝霉素B，14天后产量约为25 mg/l。因此，链霉素。YB 104被认为是第一株具有工业生产潜力的吲哚霉素菌株。
The natural products discovery program in our group utilizes deep-sea sediment-derived microorganisms and employs a bio-active guided isolation procedure and one strain many compounds (OSMAC) approach to screen bio-active natural products for practical applications in the medicinal and agricultural industry. OSMAC strategy is employed to stimulate secondary metabolite production through changing culture conditions. In this paper, we applied cultivation-dependent procedure, changing media type, leading to the discovery of a bio-active compound named inthomycin B (1) from a marine-derivedStreptomycessp. YB104. The compound was characterized based on extensive spectroscopic analyses and comparison to that in the reported literature. The quantification of inthomycin B demonstrated thatStreptomycessp. YB104 produced moderate yield of inthomycin B with a yield around 25 mg/l after 14 days. Thus,Streptomycessp. YB104 was considered to be a useful potential as a first industrial-producing strain of inthomycins.