The effect of acetylcholine-like biomimetic polymers on neuronal growth
The effect of acetylcholine-like biomimetic polymers on neuronal growth
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乙酰胆碱样仿生聚合物对神经元生长的影响
DOI：
10.1016/j.biomaterials.2011.01.044
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发表时间：
2011-04-01
期刊：
影响因子：
14
通讯作者：
Wang, Jinyi
中科院分区：
文献类型：
--
作者：
Tu, Qin;Li, Li;Wang, Jinyi
在临床需求的推动下，神经再生研究已成为组织工程研究的热点和发展方向。仿生聚合物合成和功能界面构建是诱导神经炎出芽和引导神经再生的一个很有前途的解决方案。然而，对原代海马神经元的研究很少。在这项研究中，一种新型的乙酰胆碱类仿生聚合物，他们的潜力在生物材料调制的神经再生的应用，合成使用点击化学和自由基聚合。合成的聚合物的结构包括“生物活性”单元（乙酰胆碱样单元）和“生物惰性”单元[聚（乙二醇）单元]。为了探索生物活性单元和生物惰性单元对神经元生长的影响，采用不同比例的两种初始单体聚（乙二醇）单甲基醚-甲基丙烯酸缩水甘油酯（MePEG-GMA）和甲基丙烯酸二甲氨基乙酯（DMAEMA），合成了五种不同的聚合物。采用H-1核磁共振和傅里叶变换红外光谱表征了它们的化学结构，并采用凝胶渗透色谱和差示扫描量热法测定了它们的物理性质（包括分子量、多分散性、玻璃化转变温度和熔点）。在聚合物表面上培养原代大鼠海马神经元表明，用于聚合物合成的两种初始单体的比率显著影响神经元生长。大鼠海马神经元在不同聚合物表面表现出不同的生长形态。用1：60（mol/mol）的MePEG-GMA与DMAEMA制备的聚合物表面诱导类似于聚-L-赖氨酸的神经元再生反应，聚-L-赖氨酸是神经细胞培养物的非常常见的基准材料。这些结果表明，乙酰胆碱类仿生聚合物是神经工程应用的潜在生物材料，特别是在调节海马神经元的生长。(C)2011爱思唯尔有限公司保留所有权利。
Driven by clinical needs, nerve regeneration studies have recently become the focus of research and area of growth in tissue engineering. Biomimetic polymer synthesis and functional interface construction is a promising solution to induce neuritic sprouting and guide the regenerating nerve. However, few studies have been made on primary hippocampal neurons. In this study, a new type of acetylcholine-like biomimetic polymers for their potential in biomaterial-modulated nerve regeneration application is synthesized using click chemistry and free radical polymerization. The structure of the synthesized polymers includes a "bioactive" unit (acetylcholine-like unit) and a "bioinert" unit [poly(ethylene glycol) unit]. To explore the effects of the bioactive unit and the bioinert unit on neuronal growth, different ratios of the two initial monomers poly(ethylene glycol) monomethyl ether-glycidyl methacrylate (MePEG-GMA) and dimethylaminoethyl methacrylate (DMAEMA) were employed and five different polymers were synthesized. Their chemical structures were characterized using H-1 nuclear magnetic resonance and Fourier-transform infrared spectroscopy, and their physical properties (including molecular weight, polydispersity, glass transition temperature, and melting point) were determined using gel permeation chromatography and differential scanning calorimetry. Culturing of the primary rat hippocampal neurons on the polymeric surfaces show that the ratio of the two initial monomers utilized for polymer synthesis significantly affects neuronal growth. Rat hippocampal neurons show different growth morphologies on different polymeric surfaces. The polymeric surface prepared with 1:60 (mol/mol) of MePEG-GMA to DMAEMA induces neuronal regenerative responses similar to that on poly-L-lysine, a very common benchmark material for nerve cell cultures. These results suggest that acetylcholine-like biomimetic polymers are potential biomaterials for neural engineering applications, particularly in modulating the growth of hippocampal neurons. (C) 2011 Elsevier Ltd. All rights reserved.