Somatic embryogenesis in the opium poppy, Papaver somniferum
Somatic embryogenesis in the opium poppy, Papaver somniferum
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罂粟 (Papaver somniferum) 的体细胞胚胎发生
DOI：
10.1111/j.1399-3054.1982.tb04526.x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
C. Nessler
中科院分区：
文献类型：
--
作者：
C. Nessler
本文介绍了一种在罂粟中诱导体细胞胚胎的方法。罂粟幼苗下胚轴在琼脂固化培养基上获得愈伤组织[Murashige and Skoog(1962)Physiol.种。15：473-497]含激动素0.25 mg/L(1.2μM)和萘乙酸2.0 mg/L(10.7μM)。以2.0 mg/L(9.0μM)2，4-二氯苯氧基乙酸代替NAA2.0 mg/L的液体培养基对愈伤组织进行悬浮培养。分生组织样体是紧密堆积的细胞球体，在悬浮培养物中和少数愈伤组织培养物表面发育。通过去除液体培养基中的生长调节剂，将拟分生组织分化为体细胞胚胎。胚状体形态正常，与鱼雷阶段胚胎相似，但它们在本应只含有原形成层的区域具有成熟的管状分子和乳管。通过将胚胎放在同样缺乏生长调节剂的固体培养基上，可以获得完整的植株。
A procedure is described for the induction of somatic embryos in the opium poppy. Papaver somniferum L. Callus was obtained from seedling hypocotyls on an agar solidified medium [Murashige and Skoog (1962) Physiol. Plant. 15: 473–497] containing 0.25 mg/l (1.2 μM) kinetin and 2.0 mg/l (10.7 μM) naphtalene acetic acid (NAA). Suspension cultures were initiated from callus using a liquid medium in which 2.0 mg/l (9.0 μM) 2,4-dichlorophenoxyacetic acid was substituted for NAA. Meristemoids, spheres of closely packed cells, developed in suspensions and on the surface of a few callus cultures. Differentiation of meristemoids into somatic embryos was accomplished by removing growth regulators from the liquid medium. Embryoids appeared morphologically normal and similar to torpedo stage embryos, however, they possessed mature tracheary elements and laticifers in areas that should have contained only procambium. Whole plants have been obtained by placing embryos in the light on solid medium that also lacked growth regulators.