EFFECT OF INSULIN ON HUMAN CHORIONIC-GONADOTROPIN SECRETION BY PLACENTAL EXPLANTS
EFFECT OF INSULIN ON HUMAN CHORIONIC-GONADOTROPIN SECRETION BY PLACENTAL EXPLANTS
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DOI：
10.1093/oxfordjournals.humrep.a138154
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发表时间：
1993-06-01
影响因子：
6.1
通讯作者：
SHURTZSWIRSKI, R
中科院分区：
文献类型：
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作者：
BARNEA, ER;NEUBRUN, D;SHURTZSWIRSKI, R
使用静态和动态培养模型测试生理浓度的胰岛素（5 - 50 μ U/ml）对妊娠早期（7-9周）和足月胎盘外植体的人绒毛膜促性腺激素（HCG）分泌的影响。在静态培养中，胰岛素对胎盘外植体的HCG分泌产生显著的双相抑制作用（5 μ U/ml时为80%，50 μ U/ml时为40%）。在近似空腹血浆水平下，将25 μ U/ml胰岛素加入到灌流外植体中持续大于或等于8分钟也具有快速抑制作用。用25 μ U/ml胰岛素也观察到对HCG搏动的延迟抑制作用，在过夜预孵育后HCG搏动幅度降低2倍，曲线下面积降低4倍（P < 0.01）。胰岛素对足月静态培养无影响。胰岛素样生长因子（IGF-I）和成纤维细胞生长因子（bFGF）对静态培养的HCG分泌的影响无统计学意义。总之，生理浓度的胰岛素抑制HCG分泌在孕早期胎盘在体外。这种效应是胎龄依赖性和特异性的，因为它不被IGF-I或bFGF模拟。因此，胰岛素可能是妊娠早期滋养层HCG分泌的重要调节剂。
The effect of physiological concentrations of insulin (5 - 50 muU/ml) was tested on human chorionic gonadotrophin (HCG) secretion by first trimester (7-9 weeks) and term placental explants using both static and dynamic culture models. In static cultures, insulin exerted a significant biphasic inhibitory effect (80% at 5 muU/ml and 40% at 50 muU/ml) on HCG secretion by placental explants. At approximate fasting plasma levels, 25 muU/ml insulin added to superfused explants for greater-than-or-equal-to 8 min also had a rapid inhibitory effect. A delayed inhibitory effect on HCG pulsatility was also observed using 25 muU/ml insulin, with a 2-fold decrease in HCG pulse amplitude and a 4-fold decrease in the area under the curve following overnight pre-incubation (P < 0.01). Insulin had no effect in static cultures at term. The effect of insulin-like growth factor (IGF-I) and fibroblast growth factor (bFGF) on HCG secretion in static cultures was not statistically significant. In conclusion, physiological concentrations of insulin inhibit HCG secretion in first trimester placenta in vitro. This effect is gestational age dependent and specific since it is not mimicked by IGF-I or bFGF. Thus, insulin may be an important modulator of trophoblastic HCG secretion during early pregnancy.