Radial glia is a progenitor developing of neocortical neurons in the cerebral cortex
Radial glia is a progenitor developing of neocortical neurons in the cerebral cortex
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DOI：
10.1016/s0168-0102(01)00259-0
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发表时间：
2001-09-01
影响因子：
2.9
通讯作者：
Kaneko, T
中科院分区：
文献类型：
--
作者：
Tamamaki, N;Nakamura, K;Kaneko, T
新皮层神经元是由大脑皮层脑室区的神经干细胞分裂产生的。我们研究了通过用修饰的GFP重组腺病毒感染神经上皮细胞来产生神经元。腺病毒DNA在每次细胞分裂时仅由一个子细胞遗传，并且从祖细胞单向传播到后代。由于胚胎新皮质的脑室区（VZ）表达腺病毒受体，CAR无处不在，因此可以通过腺病毒感染来揭示VZ中细胞的形态和细胞谱系。放射状胶质细胞、双极细胞和球形细胞在VZ中被发现为修饰的GFP阳性（mGFP+）。双极细胞（放射状细胞）有一个放射状突起，不与软脑膜接触，在其放射状突起的边缘有一个生长锥样结构，而放射状胶质细胞有一个跨越所有皮质层的突起。病毒感染后10小时，大多数mGFP+细胞是放射状细胞。在接下来的8小时内，mGFP+新皮质细胞中mGFP+放射状胶质细胞的百分比从18%增加到50%，而放射状/球形细胞中的百分比从75%下降到19%。放射状胶质细胞常不对称分裂，产生球形细胞和神经元前体。球形细胞似乎通过延伸放射状突起而成为放射状细胞。球形细胞、放射状细胞和放射状胶质细胞似乎构成了一个增殖的细胞周期，在此周期中产生有丝分裂后的神经元前体细胞。继承放射状突起的神经元前体细胞放射状迁移并发育成新皮层神经元。病毒感染后4天，97%的mGFP+细胞是新皮质神经元。在这里，我们提出，放射状胶质细胞是新皮层神经元的祖细胞，并且大量的放射状迁移神经元由它们自己的连接到软脑膜的放射状突起引导。(C)2001 Elsevier Science爱尔兰有限公司和日本神经科学学会。All rights reserved.
Neocortical neurons are produced by cell division of neural stem cells in the ventricular zone of the cerebral cortex. We investigated the production of neurons by infecting neuroepithelial cells with a modified GFP-recombinant adenovirus. The adenovirus DNA is inherited by only one daughter cell at each cell division and travels one way from the progenitor to the progeny. Since the ventricular zone (VZ) of the embryo neocortex expressed an adenovirus receptor, CAR ubiquitously, morphology and cell-lineage of cells in the VZ could be revealed by the adenovirus infection. Radial glias, cells with a bipolar shape, and spherical cells were found as modified-GFP-positive (mGFP+) in the VZ. The bipolar cells (radial cells) had a radial process not in contact with the pia mater and a growth-cone-like structure at the edge of their radial process, while the radial glias had a process spanning all the cortical layers. Ten hours after viral infection, most mGFP+ cells we're radial cells. In the following 8 h, the percentage of mGFP+ radial glias in mGFP+ neocortical cells increased from 18 to 50%, while that in radial/spherical cells decreased from 75 to 19%. The radial glias often divided asymmetrically and produced spherical cells and neuronal precursors. The spherical cells seemed to become radial cells by extending a radial process. The spherical cells, radial cells and radial glias seemed to constitute a proliferating cell cycle during which postmitotic neuronal precursors are produced. The neuronal precursors that inherited the radial processes migrated radially and developed into neocortical neurons. Four days after the viral infection, 97% of mGFP+ cells were neocortical neurons. Here, we propose that the radial glia is a progenitor of neocortical neurons, and that a significant number of radially migrating neurons is guided by their own radial processes connected to the pia mater. (C) 2001 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.