Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors
Fluorescence Quenching-Based Mechanism for Determination of Hypochlorite by Coumarin-Derived Sensors
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DOI：
10.3390/ijms20020281
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发表时间：
2019-01-02
影响因子：
5.6
通讯作者：
Karcz, Dariusz
中科院分区：
文献类型：
--
作者：
Starzak, Karolina;Matwijczuk, Arkadiusz;Karcz, Dariusz
通过对三种结构相似的高荧光香豆素的次氯酸盐感应效能的光谱和色谱研究，提出了一种基于荧光猝灭的次氯酸盐测定机制。发现作用模式依赖于香豆素基探针与次氯酸钠反应产生的氯化作用。重要的是，氯代衍生物的形成伴随着探针荧光强度的线性下降。结果表明，香豆素依赖的次氯酸盐识别机制适用于次氯酸盐的体外检测和定量测定。
A fluorescence quenching-based mechanism for the determination of hypochlorite was proposed based on spectroscopic and chromatographic studies on the hypochlorite-sensing potency of three structurally similar and highly fluorescent coumarins. The mode of action was found to rely upon a chlorination of the coumarin-based probes resulting from their reaction with sodium hypochlorite. Importantly, the formation of chlorinated derivatives was accompanied by a linear decrease in the fluorescence intensities of the probes tested. The results obtained suggest the applicability of a coumarin-dependent hypochlorite recognition mechanism for the detection of, as well as for quantitative determination of, hypochlorite species in vitro.