ON THE INDUCTION OF 5-LIPOXYGENASE EXPRESSION AND ACTIVITY IN HL-60 CELLS - EFFECTS OF VITAMIN-D3, RETINOIC ACID, DMSO AND TGF-BETA
ON THE INDUCTION OF 5-LIPOXYGENASE EXPRESSION AND ACTIVITY IN HL-60 CELLS - EFFECTS OF VITAMIN-D3, RETINOIC ACID, DMSO AND TGF-BETA
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DOI：
10.1006/bbrc.1994.2846
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发表时间：
1994-12-30
影响因子：
3.1
通讯作者：
STEINHILBER, D
中科院分区：
文献类型：
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作者：
BRUNGS, M;RADMARK, O;STEINHILBER, D
血清蛋白TGF β在DMSO分化的HL-60细胞中诱导5-LO活性，部分依赖于血清脂质的存在(Steinhilber， D., Hoshiko， S., Grunewald， J., Radmark， O., and Samuelsson， B. (1993) Biochim。Biophys。学报1178,1-8)。在这里，我们证明了血脂部分可以被皮摩尔浓度的1,25-二羟基维生素D3 （VD3）所取代。高浓度VD3 （24 nM）诱导完整HL-60细胞在DMSO和血清蛋白存在下分化4天后，5-LO mRNA表达量增加4倍，5-LO蛋白表达量增加14倍，5-LO活性上调38倍。此外，单独使用VD3可以显著上调5-LO蛋白的表达和活性。另一方面，单独TGF β是一个较差的5-LO通路诱导剂，需要分化诱导剂（视黄酸、DMSO或VD3）的存在。当VD3和TGF β联合作用时，对细胞匀浆和完整细胞中5-LO蛋白的表达和活性的诱导作用最为显著。(C) 1994学术出版社，Inc.
Induction of 5-LO activity in DMSO differentiated HL-60 cells, by the serum protein TGF beta, was partially dependent on the presence of serum lipids (Steinhilber, D., Hoshiko, S., Grunewald, J., Radmark, O., and Samuelsson, B. (1993) Biochim. Biophys. Acta 1178, 1-8). Here we demonstrate that the serum lipid fraction can be substituted by picomolar concentrations of 1,25-dihydroxyvitamin D3 (VD3). A high concentration of VD3 (24 nM) gave a 4-fold induction of 5-LO mRNA, a 14-fold increase in 5-LO protein, and a 38-fold upregulation of the 5-LO activity of intact HL-60 cells after differentiation in the presence of DMSO and serum proteins for 4 days.Also VD3 alone gave a substantial upregulation of 5-LO protein expression and activity. On the other hand, TGF beta alone was a poor inducer of the 5-LO pathway, the presence of a differentiation inducer (retinoic acid, DMSO or VD3) was required. The most prominent induction of 5-LO protein expression and activity in cell homogenates and intact cells was observed when VD3 and TGF beta were combined. (C) 1994 Academic Press, Inc.