CELL-SPECIFIC EXPRESSION OF THE CARROT EP2 LIPID TRANSFER PROTEIN GENE
CELL-SPECIFIC EXPRESSION OF THE CARROT EP2 LIPID TRANSFER PROTEIN GENE
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DOI：
10.1105/tpc.3.9.907
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发表时间：
1991-09-01
期刊：
影响因子：
11.6
通讯作者：
DEVRIES, SC
中科院分区：
文献类型：
--
作者：
STERK, P;BOOIJ, H;DEVRIES, SC
通过表达筛选获得了胡萝卜胚性细胞培养物分泌的10-kD蛋白的cDNA，命名为胞外蛋白2（EP 2）。 衍生的蛋白质序列和抗异源植物脂质转移蛋白的抗血清鉴定了EP 2蛋白作为脂质转移蛋白。 蛋白质凝胶印迹分析表明EP 2蛋白存在于细胞培养物的细胞壁和条件培养基中。 RNA凝胶印迹分析表明，EP 2基因表达的胚性细胞培养物，苗的茎尖，发育中的花，和成熟的种子。 原位杂交结果表明，EP 2基因在体细胞和合子胚的原皮层细胞中表达，在叶原基和所有花器官的表皮细胞中瞬时表达。 在茎尖分生组织中，在图尼卡和侧带中发现表达。 在成熟的种子中，EP 2基因在珠被的外表皮、种皮和果皮表皮中表达，以及在两种表皮之间短暂表达。 基于EP 2蛋白的胞外位置和编码基因的表达模式，我们提出了植物脂质转移蛋白在角质单体通过胞外基质运输到角质合成位点中的作用。
A cDNA corresponding to a 10-kD protein, designated extracellular protein 2 (EP2), that is secreted by embryogenic cell cultures of carrot was obtained by expression screening. The derived protein sequence and antisera against heterologous plant lipid transfer proteins identified the EP2 protein as a lipid transfer protein. Protein gel blot analysis showed that the EP2 protein is present in cell walls and conditioned medium of cell cultures. RNA gel blot analysis revealed that the EP2 gene is expressed in embryogenic cell cultures, the shoot apex of seedlings, developing flowers, and maturing seeds. In situ hybridization showed expression of the EP2 gene in protoderm cells of somatic and zygotic embryos and transient expression in epidermis cells of leaf primordia and all flower organs. In the shoot apical meristem, expression is found in the tunica and lateral zone. In maturing seeds, the EP2 gene is expressed in the outer epidermis of the integument, the seed coat, and the pericarp epidermis, as well as transiently in between both mericarp. Based on the extracellular location of the EP2 protein and the expression pattern of the encoding gene, we propose a role for plant lipid transfer proteins in the transport of cutin monomers through the extracellular matrix to sites of cutin synthesis.