Induction of P450 3A by ethylbenzene without altering RNA levels.
Induction of P450 3A by ethylbenzene without altering RNA levels.
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乙苯诱导 P450 3A 而不改变 RNA 水平。
DOI：
10.1006/bbrc.1994.2066
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发表时间：
1994
影响因子：
3.1
通讯作者：
Backes,WL
中科院分区：
文献类型：
--
作者：
Yuan,W;Cawley,GF;Eyer,CS;Backes,WL
采用玉米油中单次腹腔注射乙苯处理大鼠，检测其对细胞色素P450 3a依赖性活性、免疫反应蛋白水平和RNA水平的影响。乙苯增加P450 3a依赖性2β-羟化睾酮和免疫反应蛋白水平。这些水平在24小时后达到最高，随后逐渐降低。尽管P450 3A蛋白增加，但P450 3A1和P450 3A2 mRNA水平均未因碳氢化合物处理而改变。这些结果清楚地表明，P450同工酶可以被P450 3A蛋白的翻译激活或稳定诱导，并提示P450 3A2水平升高。
Rats were treated with a single intraperitoneal injection of ethylbenzene in corn oil and the effects on cytochrome P450 3A-dependent activities, immunoreactive protein levels and RNA levels were examined. Ethylbenzene increased both P450 3A-dependent 2β-hydroxylation of testosterone and immunoreactive protein levels. These levels were maximally induced by 24 hr and diminished thereafter. Despite the increases in P450 3A protein, neither P450 3A1 nor P450 3A2 mRNA levels were altered by treatment with the hydrocarbon. These results clearly demonstrate that this P450 isozyme can be induced by either translational activation or stabilization of P450 3A protein and are suggestive of an elevation of P450 3A2 levels.