Endoproteolytic processing of the lymphocytic choriomeningitis virus glycoprotein by the subtilase SKI-1/S1P
Endoproteolytic processing of the lymphocytic choriomeningitis virus glycoprotein by the subtilase SKI-1/S1P
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DOI：
10.1128/jvi.77.5.2866-2872.2003
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发表时间：
2003-03-01
影响因子：
5.4
通讯作者：
Lenz, O
中科院分区：
文献类型：
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作者：
Beyer, WR;Pöpplau, D;Lenz, O
淋巴细胞性脉络丛脑膜炎病毒（LCMV）的包膜糖蛋白（GP）在感染后被切割成两个亚基。我们在这里表明，这种内切蛋白水解过程是不需要运输到细胞表面，但必不可少的LCMV GP介导的假型逆转录病毒载体的感染性。通过对LCMV GP切割位点的系统突变分析，我们确定共有基序R-（R/K/H）-L-（A/L/S/T/F）（265）对于内切蛋白水解加工是必需的。与所确定的一致基序一致，我们表明细胞枯草杆菌酶SKI-1/S1 P切割LCMV GP。
The envelope glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV) is posttranslationally cleaved into two subunits. We show here that this endoproteolytic processing is not required for transport to the cell surface but is essential for LCMV GP to mediate infectivity of pseudotyped retroviral vectors. By systematic mutational analysis of the LCMV GP cleavage site, we determined that the consensus motif R-(R/K/H)-L-(A/L/S/T/F)(265) is essential for the endoproteolytic processing. In agreement with the identified consensus motif, we show that the cellular subtilase SKI-1/S1P cleaves LCMV GP.