Morphometric analysis of the extramacular retina from postmortem eyes with retinitis pigmentosa.
Morphometric analysis of the extramacular retina from postmortem eyes with retinitis pigmentosa.
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发表时间：
1999
影响因子：
4.4
通讯作者：
M. Humayun;M. Prince;E. D. Juan;Y. Barrón;Mosheh Moskowitz;I. Klock;H. Ann;Milam
中科院分区：
文献类型：
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作者：
M. Humayun;M. Prince;E. D. Juan;Y. Barrón;Mosheh Moskowitz;I. Klock;H. Ann;Milam
目的 评估死后色素性视网膜炎 (RP) 眼睛黄斑外区域内视网膜的保存程度。方法 将 18 个 RP 视网膜和 11 个年龄匹配的健康视网膜切片，通过光学显微镜进行形态测量分析。 18 个 RP 视网膜根据疾病严重程度和遗传模式进行分类。外核层 (ONL)、内核层 (INL) 和神经节细胞层 (GCL) 中的细胞核在邻近的 125 微米片段中进行计数，该区域跨越距中央凹 4 毫米至 10 毫米的区域。结果 混合效应模型显示，与对照视网膜相比，当严重程度组和遗传类型不同时，每个细胞层的平均细胞计数有所减少。中度组与重度组相比，INL和GCL中保存的细胞核数量无统计学差异。不同 RP 遗传类型的 INL 计数结果显示，与 X 连锁 (XLRP) 和单纯 RP 组相比，常染色体显性 RP (ADRP) 组保留的细胞总体平均百分比更高。对 GCL 计数的分析显示，仅 ADRP 组的 GCL 计数显着高于 XLRP 组；其他组比较均无显着性。结论 视网膜色素变性导致所有视网膜层的细胞损失，其中 ONL 损失最严重，其次是 GCL，然后是 INL。黄斑外区域中 INL 和 GCL 的保留少于先前报道的相同视网膜黄斑区域的保留。
PURPOSE To evaluate the degree of inner retinal preservation in the extramacular regions of postmortem retinitis pigmentosa (RP) eyes. METHODS Eighteen RP retinas and 11 age-matched healthy retinas were sectioned for morphometric analysis by light microscopy. The 18 RP retinas were classified by disease severity and mode of inheritance. Cell nuclei in the outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL) were counted in adjacent 125-microm segments from an area spanning the region between 4 mm and 10 mm from the fovea. RESULTS A mixed-effects model showed a decrease in mean cell counts for each of the cell layers when the severity groups and inheritance types compared with those of control retinas. There was no statistically significant difference in the number of nuclei preserved in the INL and GCL in the moderate group compared with the severe group. Results from the INL counts for the different inheritance types of RP showed a higher overall mean percentage of cells was preserved for the autosomal dominant RP (ADRP) group when compared with the X-linked (XLRP) and simplex RP groups. Analysis of the GCL counts revealed significantly more counts only in the ADRP group compared with the XLRP group; the other group comparisons were not significant. CONCLUSIONS Retinitis pigmentosa results in cell loss in all retinal layers, with the most profound loss in the ONL, followed by the GCL and then the INL. The preservation of the INL and GCL in the extramacular region is less than that previously reported for the macular region of the same retinas.