RNA TRANSSPLICING IN FLATWORMS - ANALYSIS OF TRANS-SPLICED MESSENGER-RNAS AND GENES IN THE HUMAN PARASITE, SCHISTOSOMA-MANSONI
RNA TRANSSPLICING IN FLATWORMS - ANALYSIS OF TRANS-SPLICED MESSENGER-RNAS AND GENES IN THE HUMAN PARASITE, SCHISTOSOMA-MANSONI
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DOI：
10.1074/jbc.270.37.21813
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发表时间：
1995-09-15
影响因子：
4.8
通讯作者：
SINGH, H
中科院分区：
文献类型：
--
作者：
DAVIS, RE;HARDWICK, C;SINGH, H
研究了曼氏血吸虫反式剪接的特点，以探讨扁虫剪接前导序列（SL）的意义和决定因素。只有一小部分mRNA获得SL。对30个反式剪接的mRNA和4个基因的分析显示，在基因、mRNA或其编码的蛋白质中没有可辨别的模式或共同特征，这些模式或共同特征可能解释SL添加的功能意义。虽然编码糖酵解酶烯醇化酶的mRNA是反式剪接的，但编码其他四种糖酵解酶的mRNA不是，这表明反式剪接在整个代谢途径中并不普遍。尽管扁形虫SL的3'端为mRNA提供AUG，但SL AUG通常不用于提供甲硫氨酸以用于受体mRNA中阅读框的翻译起始。SL RNA表达没有表现出明显的性别、组织或细胞特异性。反式剪接的基因经历顺式和反式剪接，并且这些各自的受体位点的序列背景非常相似。这些结果表明，扁形虫中的反式剪接最可能与SL添加赋予受体mRNA的某些性质有关，或者与初级转录本或反式剪接基因转录的某些特征有关。
Characteristics of trans-splicing in Schistosoma mansoni were examined to explore the significance and determinants of spliced leader (SL) addition in flatworms. Only a small subset of mRNAs acquire the SL. Analysis of 30 trans-spliced mRNAs and four genes revealed no discernable patterns or common characteristics in the genes, mRNAs, or their encoded proteins that might explain the functional significance of SL addition. While the mRNA encoding the glycolytic enzyme enolase is trans-spliced, mRNAs encoding four other glycolytic enzymes are not, indicating trans-splicing is not prevalent throughout this metabolic pathway. Although the 3' end of flatworm SLs contribute an AUG to mRNAs, the SL AUG does not typically serve to provide a methionine for translation initiation of reading frames in recipient mRNAs. SL RNA expression exhibits no apparent sex, tissue, or cell specificity. Trans-spliced genes undergo both cis- and trans-splicing, and the sequence contexts for these respective acceptor sites are very similar. These results suggest trans-splicing in flatworms is most Likely associated either with some property conferred on recipient mRNAs by SL addition or related to some characteristic of the primary transcripts or transcription of trans-spliced genes.