Efficacy assessment of antifungal metabolites from Chaetomium globosum No.05, a new biocontrol agent, against Setosphaeria turcica
Efficacy assessment of antifungal metabolites from Chaetomium globosum No.05, a new biocontrol agent, against Setosphaeria turcica
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新型生防剂球形毛壳菌 05 号抗真菌代谢物对抗斑球菌的功效评估
DOI：
10.1016/j.biocontrol.2012.10.005
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发表时间：
2013-01-01
影响因子：
4.2
通讯作者：
Pan, Hongyu
中科院分区：
文献类型：
--
作者：
Zhang, Guizhen;Wang, Fengting;Pan, Hongyu
北方玉米叶枯病（Northern corn leaf blight，NCLB）是由玉米大斑病菌（Setosphaeria turcica）引起的玉米叶部病害。本研究的目的是评估球毛壳菌（Cg）菌株05号的抗真菌代谢产物抑制玉米NCLB。该菌株对多种植物病原真菌特别是S. turcica在马铃薯葡萄糖琼脂培养基上生长。该菌株的次生代谢产物对菌丝生长和分生孢子萌发均有抑制作用。土耳其。当在每片8 cm长的离体叶片上共接种3滴（5 μ L/滴）分生孢子悬浮液（5 × 10（4）分生孢子/mL）时，20%培养液完全抑制北方玉米大斑病的发病率。接种后2 h培养瓶的应用。在温室研究中，NCLB对玉米幼苗的抑制率为81.9%，而在病原体接种前施用的培养物显示出更高的病害减轻率。施用该培养物对处理的玉米叶片或幼苗没有观察到影响。从提取物中分离得到两个活性成分，经光谱分析鉴定为球毛藻素A和球毛藻素C。离体和活体生物测定结果表明，球毛壳菌素A对沙门氏菌具有较好的生防效果。土耳其。据我们所知，这是第一个报告的抑制作用的评价C。globosum和球毛壳菌素A对S. turcica在离体和离体玉米叶片上的表达。(C)2012 Elsevier Inc. All rights reserved.
Northern corn leaf blight (NCLB), an important and potentially destructive corn foliar disease, is caused by Setosphaeria turcica. The intent of this study was to evaluate antifungal metabolites from Chaetomium globosum (Cg) strain No. 05 to suppress NCLB in maize. This strain significantly suppressed mycelial growth of numerous phytopathogenic fungi especially S. turcica on potato dextrose agar medium. The secondary metabolites of the strain inhibited mycelial growth and conidial germination of S. turcica. When co-inoculated at three droplets (5 mu L/droplet) of conidial suspension (5 x 10(4) conidia/mL) on each 8-cm-long detached leaf, 20% culture filtrates completely suppressed disease incidence of northern corn leaf blight. The application of the culture filtrates at 2 h post-inoculation (hpi) of S. turcica in greenhouse studies showed a 81.9% inhibition of NCLB on the seedlings, while culture filtrates applied before pathogen inoculation showed even higher rates of disease reduction. The application of the culture filtrates had no observed effects on the treated maize leaves or seedlings. Two active compounds, isolated from the extracts, were identified as chaetoglobosin A and chaetoglobosin C based on the spectroscopic analysis. Both in vitro and in planta bioassay experiments showed that chaetoglobosin A displayed potent biocontrol efficiency against S. turcica. To the best of our knowledge, this is the first report of the evaluation of the inhibitory effects of C. globosum and chaetoglobosin A against S. turcica both in vitro and on detached maize leaves. (C) 2012 Elsevier Inc. All rights reserved.