Adhesion of the probiotic strains Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 to Caco-2 cells under conditions simulating the intestinal tract, and in the presence of antibiotics and anti-inflammatory medicaments
Adhesion of the probiotic strains Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 to Caco-2 cells under conditions simulating the intestinal tract, and in the presence of antibiotics and anti-inflammatory medicaments
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DOI：
10.1007/s00203-008-0408-0
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发表时间：
2008-11-01
影响因子：
2.8
通讯作者：
Dicks, Leon M. T.
中科院分区：
文献类型：
--
作者：
Botes, Marelize;Loos, Ben;Dicks, Leon M. T.
通过荧光染色观察 Mundtii 肠球菌 ST4SA 和植物乳杆菌 423 对 Caco-2（人癌上皮）细胞的粘附情况。与干酪乳杆菌 MB1、干酪乳杆菌 Shirota、约氏乳杆菌 La1 和鼠李糖乳杆菌 GG 相比，两种菌株都显示出良好的粘附性。未发现疏水性、聚集性和对 Caco-2 细胞的粘附之间存在相关性。抗生素和抗炎药物的存在减少了细菌菌株对 Caco-2 细胞的粘附。对胃蛋白酶、胰蛋白酶和链霉蛋白酶敏感的蛋白质参与 E. mundtii ST4SA 和 L. plantarum 423 与 Caco-2 细胞的粘附。 E. mundtii ST4SA 和 L. plantarum 423 不会阻止单核细胞增生李斯特菌 ScottA 粘附到 Caco-2 细胞。含有抗菌肽 plantaricin 423 和肽 ST4SA 的菌株 ST4SA 和 423 的无细胞培养物上清液可阻止单核细胞增生李斯特菌 ScottA 侵入 Caco-2 细胞。
Adhesion of Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 to Caco-2 (human carcinoma epithelial) cells was visualized by fluorescent staining. Both strains showed good adhesion compared to L. casei MB1, L. casei Shirota, L. johnsonii La1 and L. rhamnosus GG. No correlation was found between hydrophobicity, aggregation and adhesion to Caco-2 cells. Presence of antibiotics and anti-inflammatory medicaments reduced adhesion of bacterial strains to Caco-2 cells. Proteins sensitive to pepsin, trypsin and pronase are involved in the adhesion of E. mundtii ST4SA and L. plantarum 423 to Caco-2 cells. Adhesion of Listeria monocytogenes ScottA to Caco-2 cells was not prevented by E. mundtii ST4SA and L. plantarum 423. Cell-free culture supernatants of strains ST4SA and 423, containing the antimicrobial peptides plantaricin 423 and peptide ST4SA, prevented the invasion of L. monocytogenes ScottA into Caco-2 cells.