Translational efficiency of poliovirus mRNA: mapping inhibitory cis-acting elements within the 5' noncoding region.
Translational efficiency of poliovirus mRNA: mapping inhibitory cis-acting elements within the 5' noncoding region.
复制标题
脊髓灰质炎病毒 mRNA 的翻译效率：绘制 5 非编码区内的抑制性顺式作用元件。
DOI：
10.1128/jvi.62.7.2219-2227.1988
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发表时间：
1988
影响因子：
5.4
通讯作者：
Sonenberg,N
中科院分区：
文献类型：
--
作者：
Pelletier,J;Kaplan,G;Racaniello,VR;Sonenberg,N
脊髓灰质炎病毒mRNA含有一个长约750个核苷酸的5‘非编码区(具体数字在三种病毒血清型中不同)，它包含主要启动子AUG上游的几个AUG密码子。与大多数真核mRNAs不同，脊髓灰质炎病毒的5‘端不包含m7GpppX(其中X是任何核苷酸)帽结构，并由帽非依赖性机制翻译。为了研究脊髓灰质炎病毒mRNA的表达方式，我们检测了一系列缺失突变体在mRNA的5‘非编码区的翻译效率。在这篇文章中，我们报告了不同翻译系统在改变的mRNAs被翻译的能力方面的显著差异。结果表明，脊髓灰质炎病毒5‘端非编码区存在一个抑制顺式作用元件(S)，它能抑制网织红细胞裂解物、麦胚提取物和非洲爪哇卵母细胞的转录，而不能抑制HeLa细胞提取物的转录。此外，我们还发现HeLa细胞提取物中含有一种反式作用因子(S)，可以克服这种限制。
Poliovirus mRNA contains a long 5' noncoding region of about 750 nucleotides (the exact number varies among the three virus serotypes), which contains several AUG codons upstream of the major initiator AUG. Unlike most eucaryotic mRNAs, poliovirus does not contain a m7GpppX (where X is any nucleotide) cap structure at its 5' end and is translated by a cap-independent mechanism. To study the manner by which poliovirus mRNA is expressed, we examined the translational efficiencies of a series of deletion mutants within the 5' noncoding region of the mRNA. In this paper we report striking translation system-specific differences in the ability of the altered mRNAs to be translated. The results suggest the existence of an inhibitory cis-acting element(s) within the 5' noncoding region of poliovirus (between nucleotides 70 and 381) which restricts mRNA translation in reticulocyte lysate, wheat germ extract, and Xenopus oocytes, but not in HeLa cell extracts. In addition, we show that HeLa cell extracts contain a trans-acting factor(s) that overcomes this restriction.