Vaccination with different HSV-1 glycoproteins induces different patterns of ocular cytokine responses following HSV-1 challenge of vaccinated mice
Vaccination with different HSV-1 glycoproteins induces different patterns of ocular cytokine responses following HSV-1 challenge of vaccinated mice
复制标题
DOI：
10.1016/s0264-410x(99)00056-0
复制
发表时间：
1999-06-04
期刊：
影响因子：
5.5
通讯作者：
Wechsler, SL
中科院分区：
文献类型：
--
作者：
Ghiasi, H;Hofman, FM;Wechsler, SL
我们曾报道，用不同的杆状病毒表达的HSV-1糖蛋白免疫BALB/c小鼠后，对HSV-I眼部攻击有不同程度的保护作用，从完全保护到无保护，到眼部疾病的加重。为了将特定的局部免疫反应与角膜瘢痕形成的保护和加重联系起来，我们检测了接种HSV-1疫苗的小鼠眼睛感染HSV-1后角膜中的免疫细胞渗透。小鼠接种了GO疫苗，GG可以完全防止角膜疤痕，GG对角膜疤痕没有任何保护作用，GK则会加剧角膜疤痕。在攻击后不同时间取角膜冰冻切片，检测含有IL-2、IL-4、干扰素-γ、IL-6或肿瘤坏死因子-α的细胞浸润情况。攻击前和攻击后1d，各组均未见角膜浸润性病变。免疫后3-7天，大量含有IL-3和干扰素-γ的细胞，但含有IL-2的细胞很少，已渗入GE或模拟免疫小鼠的角膜。同时，GO免疫小鼠角膜中可见大量含有IL-2的细胞，但细胞倍数很少，大量含有IL-2的细胞，但含有IL-4或干扰素-γ的细胞很少。相比之下，接种了GK病的小鼠的角膜出现了高度偏向IL-2的反应；(2)接种GG或模拟眼的小鼠的角膜出现了严重的角膜疾病，并主要产生了IL-4和干扰素-γ的细胞因子反应；以及(3)GK接种的小鼠的角膜加剧了角膜疾病，并产生了强烈的IL-2、IL-4和干扰素-γ的细胞因子反应，(C)1999 Elsevier Science Ltd.。
We previously reported that vaccination of BALB/c mice with different baculovirus expressed HSV-1 glycoproteins induced varying degrees of protection against HSV-I ocular challenge, ranging from complete protection to no protection, to exacerbation of eye disease. To correlate specific local immune responses with protection and exacerbation of corneal scarring, we examined immune cell infiltrates in the cornea after ocular HSV-1 challenge of vaccinated mice. Mice were vaccinated with go, which completely protects against corneal scarring, gG, which produces no protection against corneal scarring, or gK, which exacerbates corneal scarring. Cryostat sections of cornea were taken at different times after challenge and examined for infiltrating cells containing IL-2, IL-4, IFN-gamma, IL-6, or TNF-alpha. No corneal infiltrates were seen before challenge or 1 day after ocular challenge in any groups. By days 3-7, many cells containing IL-3 and IFN-gamma, but few cells containing IL-2, had infiltrated into the corneas of ge or mock vaccinated mice. At the same times many cells containing IL-2 but few cells times, many cells containing IL-2, but few cells containing IL-4 or IFN-gamma, were seen in the corneas of go vaccinated mice. In contrast, the corneas of mice vaccinated with gK disease and developed a response highly biased toward IL-2 responses; (2) corneas from gG or mock vaccinated eyes had significant corneal disease and developed a mostly IL-4 and IFN-gamma cytokine response; and (3) corneas from gK vaccinated mice had exacerbated corneal disease and developed strong IL-2, IL-4 and IFN-gamma cytokine responses, (C) 1999 Elsevier Science Ltd. All rights reserved.