The effect of tumor necrosis factor α (TNFα), interleukin 1β (IL1β) and interleukin 6 (IL6) on endometrial PGF2α synthesis, metabolism and release in early-pregnant pigs
The effect of tumor necrosis factor α (TNFα), interleukin 1β (IL1β) and interleukin 6 (IL6) on endometrial PGF2α synthesis, metabolism and release in early-pregnant pigs
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DOI：
10.1016/j.theriogenology.2011.07.029
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发表时间：
2012-01-01
期刊：
影响因子：
2.8
通讯作者：
Kotwica, G.
中科院分区：
文献类型：
--
作者：
Franczak, A.;Zmijewska, A.;Kotwica, G.
猪子宫和胚胎产生的细胞因子可能参与调节子宫内膜前列腺素的合成、代谢和释放。我们研究了肿瘤坏死因子α (TNF α)、白细胞介素1 β （IL1 β)和白细胞介素6 （IL6）对以下因素的影响：1）子宫内膜释放前列腺素F-2 α (PGF(2) α), 2) PGF（2) α合成终酶- PGF合成酶mRNA （PGFS mRNA）的表达，3）子宫内膜分泌PGF(2) α代谢物13,14-二氢-15-酮PGF（2) α (PGFM), 4）子宫内膜nad依赖性15-羟基前列腺素脱氢酶（15-PGDH）的存在和活性。细胞因子的作用分别在母体确认妊娠前的第10-11天和第12-13天，以及围栏着床期的第15-16天进行测定，并与循环后备母猪在相应发情周期内的作用进行比较。TNF α不影响妊娠猪和周期猪子宫内膜PGR(2) α的释放。il -1 β分别促进妊娠猪和周期猪12-13天和15-16天子宫内膜PGF(2) α的释放。il - 6增加PGF(2) α的释放，主要发生在妊娠第15-16天。妊娠第12 ~ 13天，il -1 β降低了PGFS mRNA的表达（P < 0.05），而在发情周期第12 ~ 13天和第15 ~ 16天，il -1 β、TNF α和il - 6升高了PGFS mRNA的表达（P < 0.05）。妊娠猪在妊娠周期的第12-13、15-16天以及非妊娠猪的第10-11和15-16天，il -1 β增加了PGFM的释放。妊娠第15-16天TNF - α和il - 6增加了PGFM的子宫内膜分泌。我们确定猪子宫内膜中存在nadd依赖性的15-羟基前列腺素脱氢酶（15-PGDH）。在妊娠猪中，子宫内膜15-PGDH的最高表达发生在妊娠12-13天，而在未妊娠猪中，最高表达发生在发情周期的10-11天。这些数据提供了新的证据，表明TNF α、IL1 β、IL6参与调节子宫内膜PGF(2) α的合成、释放和代谢，从而在妊娠早期保护CL或促进其消退。(C) 2012爱思唯尔公司版权所有。
Cytokines produced by the porcine uterus and embryos may be involved in the regulation of endometrial prostaglandin synthesis, metabolism, and release. We studied the effect of tumor necrosis factor alpha (TNF alpha), interleukin 1 beta (IL1 beta) and interleukin 6 (IL6) on: 1) endometrial release of prostaglandin F-2 alpha (PGF(2)alpha), 2) expression of the terminal enzyme of PGF(2)alpha synthesis - PGF synthase mRNA (PGFS mRNA), 3) secretion of PGF(2)alpha metabolite 13,14-dihydro-15-keto PGF(2)alpha (PGFM) by the endometrium and 4) presence and activity of endometrial NAD-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH). The effects of cytokines were determined on days 10-11 and days 12-13, e.g., before and during maternal recognition of pregnancy, and on days 15-16, e.g., during the pen-implantation period and compared with its effect in cyclic gilts on corresponding days of the estrous cycle. TNF alpha did not affect endometrial release of PGR(2)alpha in pregnant and cyclic pigs. IL1 beta enhanced endometrial PGF(2)alpha release on days 12-13 and 15-16 in pregnant and cyclic pigs, respectively. IL6 increased PGF(2)alpha release mainly on days 15-16 of pregnancy. Expression of PGFS mRNA was decreased by IL1 beta on days 12-13 of pregnancy (P < 0.05) and increased in response to IL1 beta, TNF alpha and IL6 on 12-13 (P < 0.05) and 15-16 (P < 0.01) of the estrous cycle. IL1 beta increased release of PGFM in gravid pigs on days 12-13, 15-16 and in non-gravid pigs 10-11 and 15-16 of the cycle. On days 15-16 of pregnancy TNF alpha and IL6 increased endometrial secretion of PGFM. We determined that in porcine endometrium NAD-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) is present. In gravid pigs, the highest expression of endometrial 15-PGDH occurred during days 12-13 of pregnancy, while in non-gravid pigs during days 10-11 of the estrous cycle. These data provide new evidence that TNF alpha, IL1 beta, IL6 are involved in the regulation of endometrial synthesis, release and metabolism of PGF(2)alpha to protect CL during early pregnancy or to facilitate its regression in cyclic females. (C) 2012 Elsevier Inc. All rights reserved.