Measurement of intestinal permeability using mannitol and lactulose in children with diarrheal diseases
Measurement of intestinal permeability using mannitol and lactulose in children with diarrheal diseases
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DOI：
10.1590/s0100-879x1999001200008
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发表时间：
1999-12-01
影响因子：
2.3
通讯作者：
Lima, AAM
中科院分区：
文献类型：
--
作者：
Barboza, MS;Silva, TMJ;Lima, AAM
The excretion ratio of lactulose/mannitol in urine has been used to assess the extension of malabsorption and impairment of intestinal permeability. The recovery of lactulose and mannitol in urine was employed to evaluate intestinal permeability in children with and without diarrhea. Lactulose and mannitol probes were measured using high-performance liquid chromatography with pulsed amperometric detection (HPLC-PAD). Two groups of solutions containing 60 mu M sugars were prepared. Group I consisted of glucosamine, mannitol, melibiose and lactulose, and group II of inositol, sorbitol, glucose and lactose. In the study of intra-experiment variation, a sample of 50 mu l from each group was submitted to 4 successive determinations. The recovered amounts and retention times of each sugar showed a variation 97%. In the study of inter-experiment variation, we prepared 4 independent samples from groups I and II at the following concentrations: 1.0, 0.3, 0.1, 0.03 and 0.01 mM. The amounts of the sugars recovered varied by
