ATP regulation of ciliary beat frequency in rat tracheal and distal airway epithelium
ATP regulation of ciliary beat frequency in rat tracheal and distal airway epithelium
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DOI：
10.1113/expphysiol.2004.028746
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发表时间：
2005-07-01
影响因子：
2.7
通讯作者：
Nakahari, T
中科院分区：
文献类型：
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作者：
Hayashi, T;Kawakami, M;Nakahari, T
利用切片制备的方法，通过视频光学显微镜测量大鼠气管和远端气道纤毛细胞的纤毛搏动频率（CBF）。在气管纤毛细胞（气管切片），ATP或2-甲硫基ATP（MeSATP）增加CBF，这是抑制苏拉明（100 μ M，嘌呤能受体的抑制剂）。离子霉素（5 μ M）或毒胡萝卜素（2 μ M）增加CBF相似。无钙溶液或添加镍（1 mM）逐渐减少CBF约25%，随后的刺激与ATP（10 μ M）增加CBF短暂。嘌呤能激动剂实验表明，ATP通过P2 X和P2 Y受体增加气管纤毛细胞的CBF。ATP可增加气管纤毛细胞内[Ca ~（2+）]（i）。然而，在远端气道睫状细胞（肺切片），ATP并没有增加CBF和[Ca 2 +]（i），尽管无Ca 2+溶液降低CBF，离子霉素（5 μ M）或毒胡萝卜素（2 μ M）增加它。此外，乙酰胆碱（100 μ M）并没有增加远端气道睫状细胞的CBF，虽然它增加了气管睫状细胞的CBF。特布他林（10 μ M）是一种选择性β（2）-肾上腺素能激动剂，可增加气管和远端气道纤毛细胞的CBF。这些观察结果表明，Ca 2+动员机制通过嘌呤或毒蕈碱受体的远端气道纤毛细胞可能是不同的气管纤毛细胞。总之，CBF增加在大鼠气管和远端气道上皮中受到不同的调节。
Ciliary beat frequency (CBF) was measured by video-optical microscopy in rat tracheal and distal airway ciliary cells using a slice preparation. In tracheal ciliary cells ( tracheal slice), ATP or 2-methylthio ATP ( MeSATP) increased CBF, which was inhibited by suramin ( 100 mu M, an inhibitor of purinergic receptor). Ionomycin ( 5 mu M) or thapsigargin ( 2 mu M) increased CBF similarly. Ca2+-free solution or addition of Ni2+ ( 1 mM) decreased CBF gradually by approximately 25% and subsequent stimulation with ATP ( 10 mu M) increased CBF transiently. The purinergic agonist experiments demonstrated that ATP increases CBF in tracheal ciliary cells via both P2X and P2Y receptors. ATP increased the intracellular calcium concentration ([Ca2+](i)) in tracheal ciliary cells. However, in distal airway ciliary cells ( lung slice), ATP did not increase CBF and [Ca2+](i), although a Ca2+- free solution decreased CBF, and ionomycin ( 5 mu M) or thapsigargin ( 2 mu M) increased it. Moreover, acetylcholine ( 100 mu M) did not increase CBF in distal airway ciliary cells, although it increased CBF in tracheal ciliary cells. Terbutaline ( 10 mu M), a selective beta(2)-adrenergic agonist, increased CBF in both tracheal and distal airway ciliary cells. These observations suggest that the Ca2+- mobilization mechanisms via purinergic or muscarinic receptors of the distal airway ciliary cell may be different from those of the tracheal ciliary cell. In conclusion, the CBF increase is differently regulated in the tracheal and distal airway epithelia of the rat.