Differential Inhibitory Effects of Actinomycin D Among Strains of Poliovirus
Differential Inhibitory Effects of Actinomycin D Among Strains of Poliovirus
复制标题
放线菌素 D 对脊髓灰质炎病毒株的不同抑制作用
作者：
F. L. Schaffer;M. Gordon
谢弗、弗雷德里克·L(加州大学伯克利分校)和玛乔里·戈登。放线菌素D对脊髓灰质炎病毒株的不同抑制作用。细菌素J。91：2309-2316。1966年-放线菌素D对脊髓灰质炎病毒株在HeLa细胞中复制的能力产生了不同的影响。以LSC-2AB为例，研究了放线菌素对LSC-2AB的明显抑制作用；以MEF1为对照，抑制作用最小。当放线菌素浓度为0.1μg/ml时即可观察到这种作用，但大多数研究中使用2.5μg/ml。这种效应的可变性部分归因于生理因素。放线菌素在LSC感染的前2小时内有效，但如果在以后的时间内存在，则效果不大。它不会阻止吸附或启动启动体。它确实阻止了LSC感染细胞中核糖核酸的合成。讨论了几种可能的作用模式，其中最吸引人的是放线菌素阻止某些细胞成分的合成，这些细胞成分的浓度对某些脊髓灰质炎病毒株的复制比其他病毒株更关键。
Schaffer, Frederick L. (University of California, Berkeley), and Marjorie Gordon. Differential inhibitory effects of actinomycin D among strains of poliovirus. J. Bacteriol. 91:2309–2316. 1966.—Actinomycin D exerted a differential effect on the ability of strains of poliovirus to replicate in HeLa cells. LSc-2ab was studied as an example of a strain markedly inhibited by actinomycin; MEF1 served as a control strain with minimal inhibition. The effect was noted at an actinomycin concentration of 0.1 μg/ml, but 2.5 μg/ml was used for most studies. Variability in the effect was attributed, in part, to physiological factors. Actinomycin was effective when present during the first 2 hr of LSc infection, but had little effect if present at later times. It did not block adsorption or initiation of ecilpse. It did block synthesis of ribonucleic acid in LSc-infected cells. Several possible modes of action are discussed, the most attractive being that actinomycin blocks synthesis of some cell component, the concentration of which is more critical for replication of some poliovirus strains than others.