Permeability of the ovarian follicle of Aedes aegypti mosquitoes.
Permeability of the ovarian follicle of Aedes aegypti mosquitoes.
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埃及埃及蚊子的卵巢卵泡的渗透性。
DOI：
10.1083/jcb.50.1.201
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发表时间：
1971-07
期刊：
影响因子：
--
通讯作者：
Spielman A
中科院分区：
文献类型：
--
作者：
Anderson WA;Spielman A
用超微结构研究了不同分子量示踪剂在埃及伊蚊静息卵巢卵泡和卵黄形成卵泡中的传代。卵泡鞘的最外层（基底膜）是一个粗糙的机械过滤器。它可以自由渗透到分子量在12000到500000之间的颗粒（即细胞色素c，过氧化物酶，血红蛋白，过氧化氢酶，铁蛋白，免疫球蛋白(IgG)-过氧化物酶，右旋糖酐铁和Thorotrast），尺寸小于110 A。像碳（300 - 500a）这样大的分子完全被排除在外。尽管蛋白质和多糖示踪剂明显容易渗透基底膜，但某些惰性颗粒（如Thorotrast、Fellows- testager Div、Fellows Mfg等）却容易渗透基底膜。Co., Inc., Detroit, michigan)的渗透速度较慢。相对于所测试的示踪剂，静止的卵泡与卵黄源性卵泡一样具有渗透性。静止卵泡或卵黄发生卵泡的卵泡上皮被狭窄的细胞间通道穿透。我们的观察表明，这些空间内衬粘多糖物质。外源性示踪剂通过基底膜后，填充这些通道，而大部分物质积聚在卵周细胞间隙。在吸血后3小时内，卵母细胞的胞饮机制大大增强。由于双示踪剂研究表明，外源化合物并没有被分离，而是被合并到同一个胞泡中，因此胞饮作用对周期细胞空间中的物质没有选择性。在卵黄形成的后期，血餐后36-48小时，卵母细胞的胞饮机制减弱。同时，细胞间通道被桥粒阻塞，卵黄膜斑块将卵母细胞和卵泡上皮分开。
The passage of tracers of various molecular weights into resting and vitellogenic ovarian follicles of Aedes aegypti mosquitoes was studied ultrastructurally. The outermost layer of the follicular sheath (the basement lamina) is a coarse mechanical filter. It is freely permeable to particles with molecular weights ranging from 12,000 to 500,000 (i.e. cytochrome c, peroxidase, hemoglobin, catalase, ferritin, immunoglobulin (IgG)-peroxidase, iron dextran and Thorotrast) that have dimensions less than 110 A. Molecules as large as carbon (300–500 A) are totally excluded. Whereas proteins and polysaccharide tracers permeate the basement lamina with apparent ease, certain inert particles (e.g. Thorotrast, Fellows-Testager Div., Fellows Mfg. Co., Inc., Detroit, Mich.) penetrate more slowly. With respect to the tracers tested, resting follicles are as permeable as vitellogenic follicles. The follicle epithelium of resting or vitellogenic follicles is penetrated by narrow intercellular channels. Our observations suggest that these spaces are lined with mucopolysaccharide material. After permeating the basement lamina, exogenous tracers fill these channels, while the bulk of material accumulates in the perioocytic space. Within 3 hr after imbibing blood, the pinocytotic mechanism of the oocyte is greatly augmented. Pinocytosis is not selective with regard to material in the perioocytic space, since double tracer studies show that exogenous compounds are not separated, but are incorporated into the same pinocytotic vesicle. During later stages of vitellogenesis, 36–48 hr after the blood-meal, the pinocytotic mechanism of the oocyte is diminished. Simultaneously, the intercellular channels become occluded by desmosomes, and the vitelline membrane plaques separate the oocyte and follicle epithelium.