EFFICIENT INDUCTION OF SUPEROVULATION IN ADULT-RATS BY PMSG AND HCG
EFFICIENT INDUCTION OF SUPEROVULATION IN ADULT-RATS BY PMSG AND HCG
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DOI：
10.1538/expanim.44.111
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发表时间：
1995-04-01
影响因子：
2.4
通讯作者：
ISHIKAWA, H
中科院分区：
文献类型：
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作者：
MUKUMOTO, S;MORI, K;ISHIKAWA, H
成年Wistar-Imamichi大鼠腹腔注射PMSG，48小时后腹腔注射hCG诱导超数排卵。 PMSG和hCG的给药剂量分别为0、75、100、150、300 iu/kg体重。 在本研究中，剂量基于iu/kg体重而不是iu/大鼠，以便于准确诱导。 获得了以下结果。 在成年大鼠（10至15周龄）中，PMSG诱导形态正常和受精卵母细胞数量增加，以及超排卵卵母细胞总数增加，剂量依赖性高达150 IU/kg。 与对照组大鼠相比，增加是显着的，但PMSG治疗只有一半的效果，在年轻的大鼠（6至9周龄），与成年大鼠相比。 这些结果表明，PMSG的敏感性随年龄的变化。 在形态正常的卵母细胞中，受精卵母细胞的比例在年轻和成年大鼠中没有显著差异，并且两组都有很高的百分比。 鉴于本研究的结果，作者建议，为了收集大量的受精卵母细胞以产生转基因大鼠，在10-15周龄的成年大鼠而不是6-9周龄的幼鼠中进行超数排卵诱导，并给予剂量为150 IU/kg的PMSG和剂量为75 IU/kg的hCG。
Adult Wistar-Imamichi rats were induced to superovulate by an i.p. injection of PMSG followed by an i.p. injection of hCG 48 hr later. The doses of PMSG and hCG administered were 0, 75, 100, 150, 300 iu/kg body weight. In this study the doses were based on iu/kg body weight rather than iu/rat to facilitate accurate induction. The following results were obtained. In the adult rats (10 to 15 weeks old) PMSG induced an increase in the number of morphologically normal and fertilized oocytes, as well as in the total number of superovulated oocytes, dose-dependently upto 150 iu/kg. The increase was significant compared with control rats, but PMSG treatment had only half this effect in young rats (6 to 9 weeks old), compared with the adult rats. These results suggest that sensitivity to PMSG changes with age. The proportion of fertilized oocytes among the morphologically normal oocytes was not significantly different in young and adult rats, and both groups had high percentages. In view of the results of this study, the authors recommend that, to collect a large number of fertilized oocytes required for generation of transgenic rats, the induction of superovulation be performed in adult rats aged 10-15 weeks rather than young rats aged 6-9 weeks and giving PMSG at a dose of 150 iu/kg and hCG at a dose of 75 iu/kg.