INDUCTION OF SELF-TOLERANCE IN T-CELLS BUT NOT B-CELLS OF TRANSGENIC MICE EXPRESSING LITTLE SELF ANTIGEN
INDUCTION OF SELF-TOLERANCE IN T-CELLS BUT NOT B-CELLS OF TRANSGENIC MICE EXPRESSING LITTLE SELF ANTIGEN
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DOI：
10.1126/science.1900950
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发表时间：
1991-03-08
期刊：
影响因子：
56.9
通讯作者：
GOODNOW, CC
中科院分区：
文献类型：
--
作者：
ADELSTEIN, S;PRITCHARDBRISCOE, H;GOODNOW, CC
在一系列表达不同血清浓度可溶性溶菌酶的转基因小鼠的T细胞和B细胞库中，对转基因编码的蛋白质——鸡卵溶菌酶的自身耐受性进行了检测。在所有表达溶菌酶的品系中，无论抗原浓度如何，T细胞均具有耐受性，而当血清溶菌酶浓度低于每毫升1.5纳克（0.1 nM）时，B细胞未出现耐受性。诱导转基因高表达可恢复B细胞耐受性。这些发现支持了这样一种假说：在某些情况下，自身免疫性疾病可能是通过绕过T细胞耐受性而产生的。
Self-tolerance to a transgene-encoded protein, hen egg lysozyme, was examined in the T and B cell repertoires of a series of lines of transgenic mice that expressed different serum concentrations of soluble lysozyme. T cells were tolerant in all lines in which lysozyme was expressed irrespective of the antigen concentration, whereas B cell tolerance did not occur when the serum lysozyme concentration was less than 1.5 nanograms per milliliter (0.1 nM). Induction of elevated transgene expression could restore B cell tolerance. These findings support the hypothesis that autoimmune disease may in some instances arise through a bypass of T cell tolerance.