Morphogenesis in the retina of a slow-developing teleost:: Emergence of the GABAergic system in relation to cell proliferation and differentiation
Morphogenesis in the retina of a slow-developing teleost:: Emergence of the GABAergic system in relation to cell proliferation and differentiation
复制标题
DOI：
10.1016/j.brainres.2007.11.065
复制
发表时间：
2008-02-15
期刊：
影响因子：
2.9
通讯作者：
Rodriguez-Moldes, Isabel
中科院分区：
文献类型：
--
作者：
Candal, Eva;Ferreiro-Galve, Susana;Rodriguez-Moldes, Isabel
γ-氨基丁酸(GABA)与细胞发育过程中的增殖和分化密切相关。本研究用免疫组织化学技术研究了鲑鱼视网膜内GABA能系统的发育及其与分化标志物[钙维A(CR)和酪氨酸羟基酶(TH)]的关系。还研究了Pax6的表达，Pax6是一种眼图蛋白，参与特定类型视网膜细胞的增殖和出现。视网膜分层始于孵化前胚胎的中央，最早的GABA能细胞出现在神经节细胞层(GCL)和内核层(INL)的内侧。孵化时，水平细胞层(HCL)内也可见GABA能细胞。在Alevins中，GABA能细胞和突起在视网膜生长后横向扩散，尽管它们没有侵入成神经母细胞的视网膜区域。CR和Pax6免疫反应阳性(Ir)细胞首先出现在GCL和INL内侧，而在INL内出现稀疏的TH-ir细胞。在幼年，GABA能细胞见于GCL、INL和HCL的内侧，而CR-ir细胞则散在分布于INL和HCL的外侧。GCL中的CR-ir和GCL和INL中的Pax6-ir细胞的一个亚群与GABA能标记共定位。这项研究提供了关于硬骨鱼视网膜GABA能系统发育的进一步比较知识，并显示了与报道的快速发育物种(如斑马鱼)的差异和相似之处，在斑马鱼中，视网膜GABA的表达在一些种群中是短暂的。(C)2007 Elsevier B.V.保留所有权利。
Gamma-aminobutyric acid (GABA) has been implicated in cell proliferation and differentiation during development. In the present study, immunohistochernical techniques were used to investigate the development of the GABAergic system in the retina of the trout and its relation to markers of differentiation [calretinin (CR), and tyrosine hydroxylase (TH)]. The expression of Pax6, an eye-patterning protein involved in the proliferation and emergence of specific retinal cell types, was also studied. Retinal layering was observed to begin centrally in prehatching embryos, as the first GABAergic cells appeared in the ganglion cell layer (GCL) and inner part of the inner nuclear layer (INL). At hatching, GABAergic cells were also observed in the horizontal cell layer (HCL). In alevins, GABAergic cells and processes spread laterally following retinal growth although they did not invade neuroblastic retinal regions. CR- and Pax6-immunoreactive (ir) cells were first seen in the GCL and the inner part of the INL, whereas sparse TH-ir cells appeared in the INL. In juveniles, GABAergic cells were observed in the GCL, inner part of the INL and HCL, whereas CR-ir cells spread to the outer part of the INL and HCL. A subset of CR-ir in the GCL and of Pax6-ir cells in the GCL and INL showed colocalization with GABAergic markers. This study provides further comparative knowledge about the development of GABAergic system of the retina in teleosts and shows differences and similarities with that reported in fast-developing species such as zebrafish, in which retinal expression of GABA was transient in some populations. (c) 2007 Elsevier B.V. All rights reserved.