Decontamination of a hard surface contaminated with Bacillus anthracis ΔSterne and B-anthracis Ames spores using electrochemically generated liquid-phase chlorine dioxide (eClO2)
Decontamination of a hard surface contaminated with Bacillus anthracis ΔSterne and B-anthracis Ames spores using electrochemically generated liquid-phase chlorine dioxide (eClO2)
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DOI：
10.1111/j.1365-2672.2011.05122.x
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发表时间：
2011-11-01
影响因子：
4
通讯作者：
Shegogue, D. A.
中科院分区：
文献类型：
--
作者：
Buhr, T. L.;Young, A. A.;Shegogue, D. A.
目的：评价电化学产生的液态二氧化氯(eClO(2))对炭疽芽胞杆菌Delta Sterne和Ames芽胞的灭活效果，比较两种芽胞形成和去污方法的成本、安全性和技术限制。方法与结果：用琼脂法和肉汤法制备芽胞，接种干燥于清洁、高压灭菌的玻璃片上。采用改良的三步法(AOAC 2008.05)和单管提取法对炭疽芽胞灭活效果进行了评价。用新鲜制备的eClO(2)在室温下1分钟内灭活孢子(7.0+/-0.5log)。用Beckman Coulter Multisizer测定eClO(2)处理后炭疽芽胞的大小。结论：eClO(2)浸泡硬表面是一种有效的炭疽芽胞杀菌剂。与AOAC 2008.05相比，肉汤产孢法和单管提取法所需时间和步骤更少，相明亮孢子率更高，孢子回收率更高，更适合于对致病孢子进行生物安全3级检测。研究的意义和影响：两种测试方法证明了eClO(2)的杀菌效果。介绍了一种新的去污剂单管萃取试验方法。
Aims: To evaluate the inactivation of Bacillus anthracis Delta Sterne and Ames spores using electrochemically generated liquid-phase chlorine dioxide (eClO(2)) and compare two sporulation and decontamination methods with regard to cost, safety and technical constraints.Methods and Results: Spores were prepared via agar and broth methods and subsequently inoculated and dried onto clean, autoclave-sterilized glass coupons. Bacillus anthracis spore inactivation efficacy was evaluated using the modified three-step method (AOAC 2008.05) and a single-tube extraction method. Spores (7.0 +/- 0.5 logs) were inactivated within 1 min at room temperature using freshly prepared eClO(2). Bacillus anthracis Delta Sterne spores decreased in size after eClO(2) treatment as measured using a Beckman Coulter Multisizer.Conclusions: eClO(2) saturation of a hard surface was an effective B. anthracis sporicide. Broth sporulation and the single-tube extraction method required less time and fewer steps, yielded a higher percentage of phase-bright spores and showed higher spore recovery efficiency compared with AOAC 2008.05, making it more amenable to biosafety level 3 (BSL3) testing of virulent spores.Significance and Impact of the Study: Two test methods demonstrated the sporicidal efficacy of eClO(2). A new single-tube extraction test protocol for decontaminants was introduced.