Development of quantitative analytical method for volatile thiol compound with LC-ESI-MS as nonvolatile derivative by integrating a thiol-specific derivatization
Development of quantitative analytical method for volatile thiol compound with LC-ESI-MS as nonvolatile derivative by integrating a thiol-specific derivatization
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DOI：
10.1093/bbb/zbab129
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发表时间：
2021-07-19
影响因子：
1.6
通讯作者：
Ohtsu, Iwao
中科院分区：
文献类型：
--
作者：
Kawano, Yusuke;Suzuki, Kengo;Ohtsu, Iwao
一般来说，挥发性硫醇由于其挥发性而难以用电喷雾离子化型LC-MS进行测定。因此，我们在此评价了通过monobromobimane对其S-bimanyl衍生化的预处理，以使其能够作为非挥发性衍生物进行检测。因此，我们成功地建立了一种简便、高效的方法，用于咖啡豆中2-呋喃甲醇（咖啡香气的挥发性硫醇增香剂）的定量分析。
Generally, volatile thiols are hard to be measured with electrospray-ionization-type LC-MS due to the volatility. Therefore, we here evaluated the pretreatment of their S-bimanyl derivatization by monobromobimane to enable the detection as nonvolatile derivative. Consequently, we successfully developed the convenient and efficient method through the quantitative analysis of 2-furanmethanethiol (volatile thiol odorant of coffee aroma) in coffee bean.