INTRACELLULAR INJECTION OF PROTEIN-KINASE INHIBITOR BLOCKS THE SEROTONIN-INDUCED INCREASE IN K+ CONDUCTANCE IN APLYSIA NEURON-R15
INTRACELLULAR INJECTION OF PROTEIN-KINASE INHIBITOR BLOCKS THE SEROTONIN-INDUCED INCREASE IN K+ CONDUCTANCE IN APLYSIA NEURON-R15
复制标题
DOI：
10.1073/pnas.79.12.3877
复制
发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
LEVITAN, IB
中科院分区：
文献类型：
--
作者：
ADAMS, WB;LEVITAN, IB
5-羟色胺诱导A. californica神经元R15;该反应由cAMP介导。研究了蛋白磷酸化在对5-羟色胺反应中的作用。将cAMP依赖性蛋白激酶的特异性抑制剂细胞内注射到神经元R15中。注射液完全阻断了马槟榔碱诱导的K+电导增加至少4 h。这种阻断是选择性的，因为细胞对多巴胺的反应没有受到抑制。该阻断是由蛋白激酶抑制剂特异性产生的，因为注射其它蛋白质（α-银环蛇毒素和牛血清白蛋白）不影响血清素反应。5-羟色胺反应在注射后5-13小时完全恢复，推测是蛋白激酶抑制剂的细胞内蛋白水解的结果。蛋白质磷酸化显然是导致神经元R15中5-羟色胺激活K+通道的过程中的必要步骤。
Serotonin induces an increase in membrane K+ conductance in A. californica neuron R15; this response is mediated by cAMP. The role of protein phosphorylation in the response to serotonin was examined. A specific inhibitor of cAMP-dependent protein kinase was injected intracellularly into neuron R15. The injection blocked the serotonin-induced increase in K+ conductance completely for at least 4 h. The blockage was selective because the cell''s response to dopamine was not inhibited. The blockage was specifically produced by protein kinase inhibitor because injection of other proteins (.alpha.-bungarotoxin and bovine serum albumin) did not affect the serotonin response. The serotonin response recovered fully 5-13 h after the injection, presumably as a result of intracellular proteolysis of the protein kinase inhibitor. Protein phosphorylation is apparently a necessary step in the process that leads to activation of K+ channels by serotonin in neuron R15.