Energy regulated enzyme and non-enzyme-based antioxidant properties of harvested organic mung bean sprouts (Vigna radiata)
Energy regulated enzyme and non-enzyme-based antioxidant properties of harvested organic mung bean sprouts (Vigna radiata)
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DOI：
10.1016/j.lwt.2019.03.023
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发表时间：
2019-06-01
影响因子：
6
通讯作者：
Yang, Hongshun
中科院分区：
文献类型：
--
作者：
Chen, Lin;Tan, Jue Tong Glenna;Yang, Hongshun
研究了有机绿豆芽的能量调节酶和非酶抗氧化特性。绿豆种子萌发6d时，H2 O2的积累量为0.04 ~ 2.09mmol kg（-1）。非酶抗氧化剂（游离氨基酸、类黄酮和总酚）和抗氧化酶协同维持氧化还原平衡。与对照组相比（第4和6天），外源性ATP提高了细胞内腺苷酸约25%。在生产初期（第2、4天），过量供能使酶活性提高12.45-38.82%，导致营养性抗氧化物质的积累。与此相反，2，4-二硝基苯酚（DNP）引起能量消耗，导致酶活性降低。DNP处理提高了天冬酰胺转化和多酚合成代谢。抗氧化剂的大量消耗导致营养不足，尽管酶系统在最后被激活。总之，正能量调节可能是一种潜在的策略，以提高收获的芽的营养价值。
The energy-regulated enzyme and non-enzyme-based antioxidant properties of organic mung bean sprouts were studied. H2O2 accumulated from 0.04 to 2.09 mmol kg(-1) in mung beans during germination for 6 d. Non-enzymatic antioxidants (free amino acid, flavonoid and total phenolics) and antioxidative enzymes maintained the redox equilibrium cooperatively. Exogenous ATP improved the intracellular adenylate by around 25% compared with that in the control (day 4 and 6). Excessive energy supply enhanced the activities of the enzymes by 12.45-38.82% at the early stage of production (day 2 and 4), which resulted in the accumulation of nutritive antioxidants. By contrast, 2, 4-dinitrophenol (DNP) caused energy depletion, resulting in lower activities of the enzymes. Asparagine transformation and polyphenol synthesis metabolisms were elevated under DNP treatment. The massive consumption of antioxidants led to nutritive deficit, although the enzymatic system was activated at the end. In summary, positive energy regulation could be a potential strategy to improve the nutritive values of harvested sprouts.