Bone development in the femoral epiphysis of mice: The role of cartilage canals and the fate of resting Chondrocytes
Bone development in the femoral epiphysis of mice: The role of cartilage canals and the fate of resting Chondrocytes
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DOI：
10.1002/dvdy.21228
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发表时间：
2007-08-01
影响因子：
2.5
通讯作者：
Fritsch, Helga
中科院分区：
文献类型：
--
作者：
Blumer, Michael J. F.;Longato, Stefano;Fritsch, Helga
在哺乳动物中，软骨管的确切作用仍在讨论中。因此，我们研究了它们在小鼠股骨远端骨骺中的发育，以确定这些管道的重要性。采用不同的方法检查导致骨形成的组织、细胞和分子事件。在出生后第5天，软骨管开始形成软骨膜凹陷。D10时，在分支末梢周围可见多个小的骨化核。最后，这些核聚集在一起，在D18处，一个大的次级骨化中心(SOC)占据了整个骨痂。软骨管细胞表达I型胶原，这是一种主要的骨相关蛋白。在根管形成过程中，紧邻根管周围的几个静止的软骨细胞呈活跃的caspase3阳性，但其他细胞被释放到根管腔内，似乎仍然活着。我们认为，软骨管细胞属于骨系，因此，它们有助于骨骺的形成。几个静止的软骨细胞被指定为死亡，但其他细胞在释放到管腔后可能分化为成骨细胞。
In mammals, the exact role of cartilage canals is still under discussion. Therefore, we studied their development in the distal femoral epiphysis of mice to define the importance of these canals. Various approaches were performed to examine the histological, cellular, and molecular events leading to bone formation. Cartilage canals started off as invaginations of the perichondrium at day (D) 5 after birth. At D 10, several small ossification nuclei originated around the canal branched endings. Finally, these nuclei coalesced and at D 18 a large secondary ossification centre (SOC) occupied the whole epiphysis. Cartilage canal cells expressed type I collagen, a major bone-relevant protein. During canal formation, several resting chondrocytes immediately around the canals were active caspase 3 positive but others were freed into the canal cavity and appeared to remain viable. We suggest that cartilage canal cells belong to the bone lineage and, hence, they contribute to the formation of the bony epiphysis. Several resting chondrocytes are assigned to die but others, after freeing into the canal cavity, may differentiate into osteoblasts.