Identification of enteroviral infection among infants and children admitted to hospital with acute gastroentritis in Ho Chi Minh City, Vietnam
Identification of enteroviral infection among infants and children admitted to hospital with acute gastroentritis in Ho Chi Minh City, Vietnam
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DOI：
10.1002/jmv.20445
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发表时间：
2005-10-01
影响因子：
12.7
通讯作者：
Ushijima, H
中科院分区：
文献类型：
--
作者：
Phan, TG;Nguyen, TA;Ushijima, H
从2002年10月至2003年9月在越南胡志明市因急性胃肠炎住院的婴儿和儿童中收集的276份粪便标本，通过RTPCR和病毒分离检测肠道病毒的存在。RT-PCR检测肠道病毒27例，检出率为9.8%。然而，只有四个肠道病毒株可以通过两个不同的细胞系CaCO 2和Vero细胞培养分离，显示出特异性的细胞病变效应（CPE）。结果清楚地表明，RT-PCR是一种敏感的，具体的检测，以调查急性胃肠炎的真正负担，由于肠道病毒在临床粪便标本。在本研究中，除五月外，全年均发现肠道病毒，而十二月的数量最多。通过测序对肠道病毒进行分子分析。结果表明，肠道病毒可分为两个不同的遗传簇（I，II），并显示出巨大的遗传多样性。根据基因分析，5'非编码区（5' NCR）序列表明，越南肠道病毒的主要存在与柯萨奇病毒（51.9%）和埃可病毒（29.6%）的最大相似性。有趣的是，两个肠道病毒的测序样本与一种称为肠道病毒74的新菌株相似。这份报告是越南首次在因急性胃肠炎入院的婴儿和儿童粪便中发现肠道病毒感染。
A total of 276 fecal specimens collected from infants and children admitted to hospital with acute gastroenteritis in Ho Chi Minh City, Vietnam from October 2002 to September 2003, were tested for the presence of enteroviruses by RTPCR and virus isolation. Enteroviruses were detected in 27 patients by RT-PCR corresponding to 9.8%. However, only four enterovirus strains could be isolated by cell culture with two different cell lines CaCo2 and Vero, showing specific cytopathic effect (CPE). The results clearly indicate that RT-PCR is a sensitive, specific assay to investigate the true burden of acute gastroenteritis due to enteroviruses in clinical fecal specimens. In the present study, enteroviruses were identified throughout the year except in May and the highest number was in December. Enteroviruses were subjected to molecular analysis by sequencing. It was found that enterovirus strains detected were classified further into two distinct genetic clusters (I, II) and demonstrated the great genetic diversity among them. Based on genetic analysis, 5' noncoding region (5' NCR) sequences suggested the predominant presence of Vietnamese enteroviruses with the greatest similarities to coxsakieviruses (51.9%) and echoviruses (29.6%). Interestingly, two of the sequenced specimens of enteroviruses were similar to a new strain called enterovirus 74. This report is the first detection of enteroviral infection in feces from infants and children admitted to hospital with acute gastroenteritis in Vietnam.