Integration of Biosynthesis and Long-Distance Transport Establish Organ-Specific Glucosinolate Profiles in Vegetative Arabidopsis
Integration of Biosynthesis and Long-Distance Transport Establish Organ-Specific Glucosinolate Profiles in Vegetative Arabidopsis
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DOI：
10.1105/tpc.113.110890
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发表时间：
2013-08-01
期刊：
影响因子：
11.6
通讯作者：
Halkier, Barbara Ann
中科院分区：
文献类型：
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作者：
Andersen, Tonni Grube;Nour-Eldin, Hussam Hassan;Halkier, Barbara Ann
虽然合成和运输防御化合物对植物生存至关重要，但对这些过程的协调知之甚少。在这里，我们调查的上方和地下的源库关系的防御化合物芥子油苷在营养拟南芥。体内饲养实验表明，芥子油苷转运蛋白1和2（GTR 1和GTR 2），这是必不可少的芥子油苷在种子中的积累，也可能参与芥子油苷之间的双向分配的根和轮虫，表明韧皮部和木质部作为其运输途径。嫁接野生型，生物合成和运输突变体表明，莲座丛和根都能够合成脂肪族和吲哚芥子油苷。短链脂肪族硫代葡萄糖苷主要来源于轮虫，而长链脂肪族硫代葡萄糖苷则主要在根和轮虫中合成。因此，我们的嫁接实验表明，在营养拟南芥，GTR1和GTR2参与双向长距离运输的脂肪族，但不是吲哚芥子油苷。我们的数据进一步表明，不同的莲座和根硫代葡萄糖苷在拟南芥的配置文件是由长距离运输和空间分离的生物合成，这表明这些过程的整合是植物在复杂的自然环境中的健身至关重要。
Although it is essential for plant survival to synthesize and transport defense compounds, little is known about the coordination of these processes. Here, we investigate the above- and belowground source-sink relationship of the defense compounds glucosinolates in vegetative Arabidopsis thaliana. In vivo feeding experiments demonstrate that the glucosinolate transporters1 and 2 (GTR1 and GTR2), which are essential for accumulation of glucosinolates in seeds, are likely to also be involved in bidirectional distribution of glucosinolates between the roots and rosettes, indicating phloem and xylem as their transport pathways. Grafting of wild-type, biosynthetic, and transport mutants show that both the rosette and roots are able to synthesize aliphatic and indole glucosinolates. While rosettes constitute the major source and storage site for short-chained aliphatic glucosinolates, long-chained aliphatic glucosinolates are synthesized both in roots and rosettes with roots as the major storage site. Our grafting experiments thus indicate that in vegetative Arabidopsis, GTR1 and GTR2 are involved in bidirectional long-distance transport of aliphatic but not indole glucosinolates. Our data further suggest that the distinct rosette and root glucosinolate profiles in Arabidopsis are shaped by long-distance transport and spatially separated biosynthesis, suggesting that integration of these processes is critical for plant fitness in complex natural environments.