Crosstalk between sex-related genes and apoptosis signaling reveals molecular insights into sex change in a protogynous hermaphroditic teleost fish, ricefield eel Monopterus albus
Crosstalk between sex-related genes and apoptosis signaling reveals molecular insights into sex change in a protogynous hermaphroditic teleost fish, ricefield eel Monopterus albus
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DOI：
10.1016/j.aquaculture.2022.737918
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发表时间：
2022-02-04
期刊：
影响因子：
4.5
通讯作者：
Yan, Taiming
中科院分区：
文献类型：
--
作者：
He, Zhi;Deng, Faqiang;Yan, Taiming
黄鳝（Monopterus albus）是一种先雌后雌的雌雄同体的合鳃型物种，它经历了从雌性到雄性的连续性变，为研究脊椎动物的连续性雌雄同体机制提供了一个有趣的模型。探讨黄鳝性别转变关键基因的表达模式及其分子机制。albus，对5个性发育阶段（卵巢、早期间性期性腺、中期间性期性腺、晚期间性期性腺和精巢）的性腺转录组进行了综合研究。总共获得107，260，808个原始读数和101，886，556个干净读数，并且在性腺组织中检测到41，216个基因。通过实时定量PCR（qRT-PCR）验证了21个差异表达基因的表达水平，发现与RNA-seq数据一致，表明RNA-seq数据可靠。此外，女性相关基因的表达随着性别变化的进展而减少，而男性相关基因则表现出相反的表达模式。值得注意的是，与性别变化密切相关的基因，包括与内吞作用，钙信号通路和其他7个通路相关的基因。特别是，凋亡信号被传递到p53网络，这可能与性别变化有关。重要的是，tgfb 3、fshr、camk 4和钙调素可能是关键基因，在性别转换过程中帮助平衡生殖细胞凋亡和增殖。这些结果揭示了性腺中与性转变和凋亡信号相关的特异分子标记，这将有助于加深我们对黄鳝雌雄同体和性转变的理解。和其他硬骨鱼。
The ricefield eel Monopterus albus is a protogynous hermaphroditic synbranchiform species that undergoes sequential sex change from female to male, offering an interesting model for study in the mechanisms of sequential hermaphroditism in vertebrates. To explore the expression patterns and molecular mechanisms of key genes underlying sex change in the ricefield eel M. albus, a comprehensive study of the transcriptomes of gonads at five sexual stages (ovary, early intersexual stage gonad, middle intersexual stage gonad, late intersexual stage gonad, and testis) was performed. In total, 107,260,808 raw reads and 101,886,556 clean reads were obtained, and 41,216 genes were detected in the gonad tissues. The expression levels of 21 differentially expressed genes were verified by quantitative real-time PCR (qRT-PCR) and found to be in accordance with the RNA-seq data, suggesting that the RNA-seq data were reliable. Furthermore, the expression of female-related genes decreased as sex change progressed, whereas male-related genes exhibited the opposite expression patterns. Notably, apoptosis-related genes had close relationships with sex change, including genes associated with endocytosis, calcium signaling pathways, and 7 other pathways. In particular, apoptosis signaling was delivered to the p53 network, which may be associated with sex change. Importantly, tgfb3, fshr, camk4 and calmodulin may be the key genes, which helps balance germ cell apoptosis and proliferation during sex change. These results reveal specific molecular signatures in the gonad that are related to sex change and apoptosis signaling, which will be useful for enhancing our understanding of sequential hermaphroditism and sex change in the ricefield eel M. albus and other teleost.