Optical nanoscopy: from acquisition to analysis.
Optical nanoscopy: from acquisition to analysis.
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DOI:
10.1146/annurev-bioeng-071811-150025
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发表时间:
2012
影响因子:
9.7
通讯作者:
Bewersdorf J
中科院分区:
文献类型:
--
作者:
Gould TJ;Hess ST;Bewersdorf J
Recent advances in far-field microscopy have demonstrated that fluorescence imaging is possible at resolutions well below the long-standing diffraction limit. By exploiting photophysical properties of fluorescent probe molecules, this new class of methods yields a resolving power that is fundamentally diffraction unlimited. Although these methods are becoming more widely used in biological imaging, they must be complemented by suitable data analysis approaches if their potential is to be fully realized. Here we review the basic principles of diffraction-unlimited microscopy and how these principles influence the selection of available algorithms for data analysis. Furthermore, we provide an overview of existing analysis strategies and discuss their application.
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影响因子:
14.8
作者:
Gould, Travis J.;Verkhusha, Vladislav V.;Hess, Samuel T.
通讯作者:
Hess, Samuel T.
影响因子:
48
作者:
Gould, Travis J.;Gunewardene, Mudalige S.;Gudheti, Manasa V.;Verkhusha, Vladislav V.;Yin, Shu-Rong;Gosse, Julie A.;Hess, Samuel T.
通讯作者:
Hess, Samuel T.
影响因子:
48
作者:
Biteen, Julie S.;Thompson, Michael A.;Tselentis, Nicole K.;Bowman, Grant R.;Shapiro, Lucy;Moerner, W. E.
通讯作者:
Moerner, W. E.
影响因子:
56.9
作者:
Bates, Mark;Huang, Bo;Zhuang, Xiaowei
通讯作者:
Zhuang, Xiaowei
DOI:
10.1073/pnas.0401638101
发表时间:
2004-04-27
影响因子:
11.1
作者:
Gordon, MP;Ha, T;Selvin, PR
通讯作者:
Selvin, PR