Anatomy of herpes simplex virus DNA. II. Size, composition, and arrangement of inverted terminal repetitions
Anatomy of herpes simplex virus DNA. II. Size, composition, and arrangement of inverted terminal repetitions
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单纯疱疹病毒 DNA 的解剖。
DOI：
10.1128/jvi.15.6.1487-1497.1975
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发表时间：
1975
影响因子：
5.4
通讯作者：
B. Roizman
中科院分区：
文献类型：
--
作者：
S. Wadsworth;R. Jacob;B. Roizman
对自退火完整单链和部分变性分子的电子显微镜研究表明，单纯疱疹病毒 1 DNA 由两个不相等的区域组成，每个区域均以反向冗余序列为界。因此，区域 L（DNA 轮廓长度的 70%）将左末端区域 a1b 与其反向重复序列 b'a'1 分开，每个区域包含 6% 的 DNA。 S 区（DNA 的 9.4%）将右侧末端区域 cas（DNA 的 4.3%）与其反向重复序列 a'sc' 分开。两个末端的反转和重复的区域在拓扑上是不同的。因此，cas 富含鸟嘌呤和胞嘧啶，而只有 a1b 区域末端 1%（称为亚区 a1）富含鸟嘌呤和胞嘧啶。
Electron microscope studies on self-annealed intact single strands and on partially denatured molecules show that herpes simplex virus 1 DNA consists of two unequal regions, each bounded by inverted redundant sequences. Thus the region L (70 percent of the contour length of the DNA) separates the left terminal region a1b from its inverted repeat b'a'1, each of which comprises 6 percent of the DNA. The region S (9.4 percent of DNA) separates the right terminal region cas (4.3 percent of the DNA) from its inverted repeat a'sc'. The regions of the two termini which are inverted and repeated itnernally differ in topology. Thus, cas is guanine plus cytosine rich, whereas only the terminal 1 percent of the a1b region, designated as subregion a1, is guanine plus cytosine rich.