Endothelial proliferation in the delayed hypersensitivity reaction: an autoradiographic study.
Endothelial proliferation in the delayed hypersensitivity reaction: an autoradiographic study.
复制标题
迟发型超敏反应中的内皮增殖：放射自显影研究。
DOI：
10.4049/jimmunol.118.2.529
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发表时间：
1977
影响因子：
4.4
通讯作者：
Milton M. Sholley
中科院分区：
文献类型：
--
作者：
Peter J. Polverini;R. Cotran;Milton M. Sholley
研究了豚鼠皮肤延迟性超敏反应中内皮细胞增殖的时间顺序。小鼠对结核菌素（PPD）和二硝基氯苯（DNCB）致敏，并在刺激后12小时至7天检查皮肤病变。献祭前30分钟腹腔注射3h -胸腺嘧啶（3H-T），在1.5 mu厚的甲基丙烯酸酯切片上进行自显像评估内皮标记。内皮标记在攻毒后24小时（PPD）和12小时（DNCB）出现，在PPD致敏动物中在48至72小时达到峰值，在DNCB致敏动物中在36至48小时达到峰值。峰标指数分别为7.88和15.08%。非致敏对照组的标记指数均小于1%。内皮标记在发生时间、分布和大小上与病变的单核细胞浸润有关。因此，这些发现证明了延迟超敏反应中内皮细胞显著增殖的发生，并表明导致超敏反应表达的事件也导致内皮细胞复制。
The time-sequence of endothelial proliferation in the delayed hypersensitivity reaction was examined in the skin of guinea pigs. Animals were sensitized to tuberculin (PPD) and dinitrochlorobenzene (DNCB) and skin lesions were examined 12 hr to 7 days after challenge. Thirty minutes before sacrifice 3H-thymidine (3H-T) was injected i.p. and endothelial labeling was assessed in autoradiographs of 1.5-mu thick methacrylate sections. Endothelial labeling became manifest at 24 hr (PPD) and 12 hr (DNCB) after challenge, reaching a peak at 48 to 72 hr in PPD-sensitized animals and 36 to 48 hr in DNCB-sensitized animals. Peak labeling indices were 7.88 and 15.08%, respectively. Labeling indices for nonsensitized control groups were always less than 1%. Endothelial labeling was associated, in time of occurrence, distribution, and magnitude with mononuclear cell infiltration of the lesions. These findings thus document the occurrence of significant endothelial cell proliferation in delayed hypersensitivity responses and suggest that the events leading to the expression of hypersensitivity also result in endothelial replication.