Multiple organellar RNA editing factor (MORF) family proteins are required for RNA editing in mitochondria and plastids of plants
Multiple organellar RNA editing factor (MORF) family proteins are required for RNA editing in mitochondria and plastids of plants
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DOI：
10.1073/pnas.1202452109
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发表时间：
2012-03-27
影响因子：
11.1
通讯作者：
Brennicke, Axel
中科院分区：
文献类型：
--
作者：
Takenaka, Mizuki;Zehrmann, Anja;Brennicke, Axel
开花植物质体和线粒体中的RNA编辑将数百种选定的胞苷改变为尿苷，主要是在mRNA的编码区。编辑位点周围的特定序列可能被多达200个五肽重复（PPR）蛋白识别。这里确定的多个细胞器RNA编辑因子（MORF）蛋白家族提供了两种植物细胞器中RNA编辑机制的额外组件。质体中的编辑需要两种MORF蛋白;至少两种是线粒体中编辑所必需的。MORF蛋白的丢失消除或降低了多个位点的编辑，其中许多位点由PPR蛋白单独处理。在质体中，这两种MORF蛋白几乎在所有位点都需要完全编辑，这表明了异二聚体复合物。在酵母双杂交和下拉测定中，MORF蛋白可以连接形成异二聚体和同二聚体。此外，MORF蛋白选择性地与PPR蛋白相互作用，在植物细胞器中建立比以前认为的更复杂的编辑体。
RNA editing in plastids and mitochondria of flowering plants changes hundreds of selected cytidines to uridines, mostly in coding regions of mRNAs. Specific sequences around the editing sites are presumably recognized by up to 200 pentatricopeptide repeat (PPR) proteins. The here identified family of multiple organellar RNA editing factor (MORF) proteins provides additional components of the RNA editing machinery in both plant organelles. Two MORF proteins are required for editing in plastids; at least two are essential for editing in mitochondria. The loss of a MORF protein abolishes or lowers editing at multiple sites, many of which are addressed individually by PPR proteins. In plastids, both MORF proteins are required for complete editing at almost all sites, suggesting a heterodimeric complex. In yeast two-hybrid and pull-down assays, MORF proteins can connect to form hetero- and homodimers. Furthermore, MORF proteins interact selectively with PPR proteins, establishing a more complex editosome in plant organelles than previously thought.