Evaluating Ion Accumulation and Storage in Traveling Wave Based Structures for Lossless Ion Manipulations.
Evaluating Ion Accumulation and Storage in Traveling Wave Based Structures for Lossless Ion Manipulations.
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评估基于行波的结构中的离子积累和存储，以实现无损离子操作。
DOI：
10.1021/jasms.3c00348
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发表时间：
2023
影响因子：
3.2
通讯作者：
Ibrahim,YehiaM
中科院分区：
文献类型：
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作者：
Kwantwi-Barima,Pearl;Garimella,SandilyaVB;Attah,IsaacK;Ibrahim,YehiaM
无损离子操纵结构（SLIM）技术已经证明了高分辨率的离子迁移率分离和将复杂离子操纵集成到单个实验平台中的灵活性。为了实现IMS分离，在注入用于分离的包之前在SLIM（或SLIM内）内捕获/积聚离子提供了操作的简易性并且减少了对SLIM外部的专用离子阱的需要。为了充分表征离子积累过程，我们已经评估了TW振幅、离子收集时间和存储时间对“在SLIM”积累过程的影响。该研究利用SLIM模块，该模块包括5个不同的轨道，每个轨道具有特定的离子累积配置。研究了TW条件对3-肽混合物的累积过程的影响：肯普肽、血管紧张素II和神经降压素，TW速度为106 m/s。离子积累时间/收集时间和存储时间的影响进行了研究，除了TW振幅。总的来说，当离子收集时间从49 ms增加到163 ms时，分析物离子的信号增加，但由于空间电荷效应，当离子收集时间进一步增加到652 ms时，分析物离子的信号降低。在高TW振幅下观察到离子损失（例如，15 Vp-p和20 Vp-p）。此外，在空间电荷条件下（例如，收集时间为163和652 ms），对于施加到捕获区域的所有TW振幅，分析物离子的信号随着存储时间的增加而降低。对于离子积累，数据表明，必须利用更温和的TW条件，以最大限度地减少离子损失和碎片，以受益于“在SLIM”积累过程。较宽的SLIM轨道比较窄的轨道提供更好的性能。
Structures for lossless ion manipulations (SLIM) technology has demonstrated high resolving power ion mobility separation and flexibility to integrate complex ion manipulations into a single experimental platform. To enable IMS separations, trapping/accumulating ions inside SLIM (or in-SLIM) prior to injection of a packet for separations provides ease of operation and reduces the need for dedicated ion traps external to SLIM. To fully characterize the ion accumulation process, we have evaluated the effect of TW amplitudes, ion collection times, and storage times on the “in-SLIM” accumulation process. The study utilized a SLIM module comprising 5 distinct tracks, each with a specific ion accumulation configuration. The effect of the TW conditions on the accumulation process was investigated for a 3-peptide mixture: kemptide, angiotensin II, and neurotensin at a TW speed of 106 m/s. The effect of ion accumulation time/collection time and storage time was investigated, in addition to TW amplitude. Overall, the signal of the analyte ions increased when the ion collection time increased from 49 to 163 ms but decreased when the ion collection time increased further to 652 ms due to the space charge effects. Ion losses were observed at high TW amplitudes (e.g., 15 Vp–pand 20 Vp–p). In addition, under space charge conditions (e.g., collection times of 163 and 652 ms), the signal of the analyte ions decreased with an increase in storage times for all TW amplitudes applied to the trapping region. For ion accumulation, the data indicate that gentler TW conditions must be utilized to minimize ion losses and fragments to benefit from the “in-SLIM” accumulation process. Wider SLIM tracks provided better performance than those with narrower tracks.