ULTRASTRUCTURE OF NEUROMUSCULAR-JUNCTIONS IN DROSOPHILA - COMPARISON OF WILD-TYPE AND MUTANTS WITH INCREASED EXCITABILITY
ULTRASTRUCTURE OF NEUROMUSCULAR-JUNCTIONS IN DROSOPHILA - COMPARISON OF WILD-TYPE AND MUTANTS WITH INCREASED EXCITABILITY
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DOI：
10.1002/neu.480240804
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发表时间：
1993-08-01
期刊：
影响因子：
--
通讯作者：
BUDNIK, V
中科院分区：
文献类型：
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作者：
JIA, XX;GORCZYCA, M;BUDNIK, V
果蝇幼虫体壁的腹侧纵向肌肉由至少四种类型的突触末端支配，这些突触末端可以在光学显微镜水平上的形态学基础上进行区分。先前已证明这些肌肉的神经支配受神经元活动的调节。在本报告中，我们通过使用连续切片研究了突触波顿差异的超微结构基础，并检查了兴奋性增加的突变体中突触末端的结构。我们报告说，个体可识别的肌纤维由含有两到三种类型的突触纽扣的末端支配，这些突触纽扣可以根据突触小泡群、突触前和突触后特化以及一般形状来区分。我们提出了一个模型来解释在光学显微镜水平上观察到的布顿类型。我们发现，在过度兴奋的突变体 eag Sh 中，突触按钮处存在显着的超微结构变化。这些改变包括两种类型突触小泡的部分耗尽和第三种类型突触小泡外观的变化、突触密度的数量和外观的变化以及多泡体的存在。我们的结果表明，神经元兴奋性的增加会对突触末端结构产生深远的影响。 (C) 1993 约翰威利父子公司
The ventral longitudinal muscles of the Drosophila larval body wall are innervated by at least four types of synaptic terminals that can be distinguished on morphological grounds at the light microscopical level. The innervation of these muscles has been previously shown to be regulated by neuronal activity. In this report we investigate the ultrastructural basis for synaptic bouton differences by using serial sections, and examine the structure of synaptic terminals in mutants with increased excitability. We report that individual identifiable muscle fibers are innervated by terminals containing two to three types of synaptic boutons that can be distinguished in terms of synaptic vesicle population, presynaptic and postsynaptic specialization, and general shape. We propose a model to account for the bouton types observed at the light microscopical level. We find that in the hyperexcitable mutant eag Sh, there are dramatic ultrastructural alterations at synaptic boutons. These alterations include a partial depletion of two types of synaptic vesicles and a change in appearance of a third type, changes in number and appearance of synaptic densities, and the presence of multivesicular bodies. Our results show that an increase in neuronal excitability produces profound effects in synaptic terminal structure. (C) 1993 John Wiley & Sons, Inc.